Agenda Starmerella bombicola and sophorolipids 02 Integrated - PowerPoint PPT Presentation

01 Introduction Agenda Starmerella bombicola and sophorolipids 02 Integrated bioprocess design: a case study for Starmerella bombicola Strain engineering, process development & -scale up and application research

01. Introduction Starmerella bombicola and sophorolipids

01. Introduction: Starmerella bombicola Source: www.first-nature.com vvv Starmerella bombicola Source: www.wildlifetrusts.org

01. Introduction: Starmerella bombicola vvv Starmerella bombicola

01. Introduction: Starmerella bombicola vvv Starmerella bombicola

01. Introduction: SLs: commercial reality F e r m e n t a t i o n >> Sophorolipids Glycolipids ( M i c r o b i a l ) Source: www.wheatoleo.com • Produced by yeasts • Low foaming surfactant Source: www.rewoferm.evonik.com • Mild antimicrobial properties (anti acne, anti odor,…), vvv • Antiviral, - cancer, … • Applications: detergents and cosmetics Source: www.ecover.com Source: www.athinarom.gr

01. Introduction: Sophorolipids a complex mix • Acidic or lactonic form v

01. Introduction: Sophorolipids a complex mix • Acidic or lactonic form • Acetylation: 0, 1 or 2 groups v

01. Introduction: Sophorolipids a complex mix • Acidic or lactonic form • Acetylation: 0, 1 or 2 groups • Fatty acid chain length: C16-C18 • Unsaturated bounds: 0, 1 or 2 v

01. Introduction: Sophorolipids a complex mix • Acidic or lactonic form • Acetylation: 0, 1 or 2 groups • Fatty acid chain length: C16-C18 • Unsaturated bounds: 0, 1 or 2 • Position of hydroxylation: ω of ω-1 v

01. Introduction: Sophorolipids a complex mix • Acidic or lactonic form • Acetylation: 0, 1 or 2 groups • Fatty acid chain length: C16-C18 • Unsaturated bounds: 0, 1 or 2 • Position of hydroxylation: ω of ω-1 “Variation is desired across (1) products, v preferably not IN (2) products”

01. Introduction: disadvantages of sophorolipids Solution : Integrated Bio process Design to bring IB to market Sophorolipids Market demands Uniform Pure vvv ... Variation Cheap

02. Integrated bioprocess design: a case study for S. bombicola Genetic engineering, process development & scale up and application research

02. Integrated bioprocess design approach (IBD) A case study for BS production with S. bombicola Process development & scale Genetic Engineering up vvv Uniform Uniform (New-to-nature) sophorolipids Pure Variation Cheap Cheap

02. Integrated bioprocess design approach (IBD) A case study for BS production with S. bombicola Genetic Engineering vvv Uniform (New-to-nature) sophorolipids Variation Cheap

02. IBD approach: strain engineering S. bombicola wild type v sophorolipids

02. IBD approach: strain engineering Increasing uniformity: 100 % v Battery of new S. bombicola strains 100 % Lactonic SLs Acidic SLs

02. 02. IBD approach: strain engineering Increasing variety vvv

02. Integrated bioprocess design approach (IBD) A case study for BS production with S. bombicola Genetic Engineering vvv Uniform (New-to-nature) sophorolipids Variation Cheap

02. Integrated bioprocess design approach (IBD) A case study for BS production with S. bombicola Process development & scale Genetic Engineering up vvv Uniform Uniform (New-to-nature) sophorolipids Pure Variation Cheap Cheap

02. IBD approach: Process Development 02. vvv

02. IBD approach: Process Development Bolaform Overall productivity lower as wild type Sophorolipids vvv

02. IBD approach: Process Development Bolaform Overall productivity lower as wild type Sophorolipids • Strain improvement vvv

02. IBD approach: Process Development Bolaform Overall productivity lower as wild type Sophorolipids • Strain improvement • Process development vvv

02. IBD approach: Process Development Bolaform Overall productivity lower as wild type Sophorolipids • Strain improvement • Process development FERMENTATION: ° Standard conditions (0.05 g/L.h) vvv ° Medium optimization (0.22 g/L.h) ° Substrate optimization (0.44 g/L.h) ° Fermentation optimization (0.72 g/L.h)

02. IBD approach: Process Development Bolaform Overall productivity lower as wild type Sophorolipids • Strain improvement • Process development DOWNSTREAM PROCESSING: ° Purity (85 % to 95 %) vvv ° Yield/Recovery (70 % to 98 %)

02. IBD approach: Scale up Bolaform Overall productivity lower as wild type Sophorolipids • Strain improvement • Process development • Scale up 7 L => 100 L => 15 000 L: vvv

02. IBD approach: Scale up vvv

02. IBD approach: Scale up Bolaform • Strain improvement Sophorolipids • Process development • Scale up 7 L => 100 L => 15 000 L: ° Generate data (TEA and LCA) vvv

02. IBD approach: Scale up Bolaform • Strain improvement Sophorolipids • Process development • Scale up 7 L => 100 L => 15 000 L: ° Generate data (TEA and LCA) vvv ° Generate samples (kg to ton scale)

02. IBD approach: Application research Bolaform • Strain improvement Sophorolipids • Process development • Scale up • Application research vvv

02. IBD approach: Application research 52% H o u s e h o l d d e t e r g e n t s 19% O t h e r s ( p a i n t s & i n k s , o i l f i e l d c h e m i c a l s , t e x t i l e s , . . . ) v P e r s o n a l C a r e 12% I n d u s t r i a l c l e a n e r s 7% F o o d p r o c e s s i n g 6% A g r i c u l t u r a l c h e m i c a l s 4%

02. IBD approach: Application research 52% H o u s e h o l d d e t e r g e n t s 19% O t h e r s ( p a i n t s & i n k s , o i l f i e l d c h e m i c a l s , t e x t i l e s , . . . ) v P e r s o n a l C a r e 12% I n d u s t r i a l c l e a n e r s 7% F o o d p r o c e s s i n g 6% A g r i c u l t u r a l c h e m i c a l s 4%

02. IBD approach: Application research Bolaform • Strain improvement Sophorolipids • Process development • Scale up • Application testing ° Emulsification vvv ° Wetting ability ° Film formation / contact angle ° Anti scaling properties ° Foaming ° CMC ° Stability

02. IBD approach: Application research Bolaform • Strain improvement Sophorolipids • Process development • Scale up • Application testing ° Emulsification vvv ° Wetting ability ° Film formation / contact angle ° Anti scaling properties ° Foaming ° CMC ° Stability

02. IBD approach: Application research Bolaform • Strain improvement Sophorolipids • Process development • Scale up • Application testing ° Emulsification vvv ° Wetting ability ° Film formation / contact angle ° Anti scaling properties ° Foaming ° CMC ° Stability

02. IBD approach: Application research vvv Stability

02. IBD approach: Application research Bolaform • Strain improvement Sophorolipids • Process development • Scale up • Application testing ° Emulsification vvv ° Wetting ability ° Film formation / contact angle ° Anti scaling properties ° Foaming ° CMC ° Stability

02. IBD approach: Application research Bolaform Stability issues with bola sophorolipids Sophorolipids • Strain improvement ° create a new strain producing stable bola’s vvv

02. IBD approach: Application research Bolaform Stability issues with bola sophorolipids Sophorolipids • Strain improvement ° create a new strain producing stable bola’s vvv

02. IBD approach: an iterative process IBD approach to bring bola SLs to market IPD-to bring microbial biosurfactants to market Strain Fermentation Purification engineering Development Development v Application 1 SCALE UP Application 2 Application 4 Application 3 COMMERCIALISATION Of BOLA SOPHOROLIPIDS

02. IBD approach: projects ° FP7 project Biosurfing ° FP7 project IB2Market + ° IWT-IM project Stabosurf vvv ° BBI-H2020 project Carbosurf ° Vlaio Intercluster project AppliSurf ° H2020 project Marisurf

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