A New genera tjon of hy giene moni t oring LuciPac A3 Importør: Impex Produkter AS Gamle Drammensvei 107 1363 HØVIK Tel. 22 32 77 20 info@impex.no www.impex.no 1
What’s ATP （ Adenosine TriPhosphate ） ? ATP is the universal energy molecule found in all living things. ATP ATP ATP ATP ATP ATP Food Residues Microorganisms ATP is an indicator ATP ATP of uncleanness and ATP ATP cleaning efficacy. 2
ATP Rapid Hygiene Monitoring ・ Invisible uncleanness can be quantified in “Anytime”, “Anywhere” and by “Anyone” in 30 sec. - Uncleanness record management - Enhancing employee’s awareness of hygiene ・ Risk for both food poisoning and allergen contamination can be checked at the same time. 3
ATP Hygiene Monitoring ATP : Adenosine triphosphate ✓ ATP is a molecule found in all living cells and serves as an indicator of overall surface hygiene and cleaning efficacy ✓ ATP is easily converted into ADP and/or AMP by biological metabolism or by decomposition at high temperature and pH ATP ADP AMP Decomposition of ATP derivatives Why A3 assay (ATP+ADP+AMP)? A3 detects ATP+ADP+AMP offering higher sensitivity to find microorganisms and biofilms that others miss. Just as fast and easy to use as conventional tests, but its advanced chemistry produces superior detection to uncover contaminants that you 4 may be missing.
Stability of ATP ✓ ATP is easily decomposed into ADP and AMP ✓ A3 assay provides consistent results and high sensitivity Storage condition : 80 ºC (176 ºF) Detection : ● ATP ● ADP ● AMP ● A3 assay (ATP+ADP+AMP) 5
Recycling A3 assay by Kikkoman Technology Luciferase D-luciferin Oxyluciferin Mg 2+ + O 2 + PPi + CO 2 PK ATP ADP AMP AMP Mg 2+ Pyruvate PEP Mg 2+ PEP Pyruvate + PPi PPDK + Pi PK: pyruvate kinase PEP: phosphoenolpyruvate PPi: pyrophosphate PPDK: pyruvate orthophosphate dikinase 6
Players on ATP Hygiene Monitoring Test ✓ All players except for Kikkoman detect ATP alone ✓ A3 is able to detect all ATP derivatives (ATP+ADP+AMP) BioControl Kikkoman 3M Hygiena Neogen Charm Instruments Devices ATP Detection +ADP ATP ATP ATP ATP ATP Targets +AMP 7
ATP vs A3 ~Meat and Egg~ 豚肉（生） 牛肉（生） 鶏ムネ肉 Chicken (Raw) Beef (Raw) Pork (Raw) 2,500 4,000 5,000 2,000 4,000 3,000 1,500 3,000 2,000 Relative Light Units (RLU) 1,000 2,000 1,000 500 1,000 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 ATP A3 ベーコン ソーセージ 生卵 Whole egg (Raw) Bacon Sausage 500,000 30,000 1,200 400,000 900 20,000 300,000 600 200,000 10,000 300 100,000 0 0 0 ATP A3 ATP A3 ATP A3 A3 is a good index of meat & egg residues 8
ATP vs A3 ~Seafood~ マグロ 鯛生 サーモン生 Sea bream (Raw) Tuna (Raw) Salmon (Raw) 4,000 10,000 3,000 3,000 7,500 2,000 2,000 5,000 Relative Light Units (RLU) 1,000 1,000 2,500 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 ATP A3 ATP A3 ツナ缶 エビ 生カキ Tuna (Canned) Oyster (Raw) Shrimp (Raw) 50,000 500,000 70,000 60,000 400,000 40,000 50,000 300,000 30,000 40,000 30,000 20,000 200,000 20,000 10,000 100,000 10,000 0 0 0 ATP A3 ATP A3 ATP A3 A3 is a good index of seafood residues 9
ATP vs A3 ~Dairy~ 牛乳 ホイップクリーム ヨーグルト Milk (Raw) Yogurt Whipped Cream 400 200 30,000 300 150 20,000 Relative Light Units (RLU) 200 100 10,000 100 50 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 アイス チェダー チーズ Camembert cheese Cheddar cheese Ice Cream 800 150 70,000 60,000 600 50,000 100 40,000 400 30,000 50 20,000 200 10,000 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 A3 is a good index of dairy residues 10
ATP vs A3 ~Nuts~ カシュー アーモンド ピーナッツ Peanuts Cashew Almond 16,000 16,000 16,000 12,000 12,000 12,000 8,000 Relative Light Units (RLU) 8,000 8,000 4,000 4,000 4,000 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 ATP A3 ピスタ くるみ ヘーゼル Pistachio Hazel nuts Walnuts 40,000 4,000 12,000 30,000 3,000 8,000 20,000 2,000 4,000 10,000 1,000 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 ATP A3 A3 is a good index of nuts residues 11
ATP vs A3 ~Fruits & Vegetables~ オレンジ トマト バナナ Tomato Banana Orange 16,000 30,000 12,000 12,000 20,000 8,000 Relative Light Units (RLU) 8,000 10,000 4,000 4,000 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 ATP A3 ポテチ 食パン 白ごま Sesame Potato chips Soybeans 50,000 5,000 30,000 4,000 40,000 20,000 3,000 30,000 2,000 20,000 10,000 10,000 1,000 0 0 0 ATP A3 ATP A3 ATP A3 A3 is a good index of fruits & vegetables residues. 12
ATP vs A3 ~Fermented foods & fungi~ 酵母エキス ビール 食パン Yeast extract Beer Bread 100,000 4,000 5,000 4,000 75,000 3,000 3,000 Relative Light Units (RLU) 50,000 2,000 2,000 25,000 1,000 1,000 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 マッシュ チョコレート ワイン酢 Mushroom Wine vinegar Chocolate 20,000 5,000 500 4,000 400 15,000 3,000 300 10,000 200 2,000 5,000 1,000 100 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 A3 is a good index of fermented foods & fungi residues. 13
Kikkoman A3 vs. Hygiena vs. 3M in food Data shows A3 has higher sensitivity on detection of food residue than either Hygiena or 3M 14
A3 vs. Hygiena vs. 3M in Thai Beer Luminescence Intensity (RLU) Data shows A3 has higher sensitivity to beer than either Hygiena or 3M 15
Sanitation verification by ATP, ADP, and AMP Detergent Detergent Hot water Before Water washing washing washing washing washing (1 st ) (2 nd ) 200RLU Detection of ATP alone is not enough for sanitation monitoring 16
Superiority of A3 Microorganism ATP A3 Excellent Excellent Food residue Fair Excellent Fair Excellent 17
Superiority of A3 Microorganism ATP A3 Excellent Excellent Food residue Fair Excellent A3 can also detect breeding ground of bacteria Fair Excellent 18
A3 vs Protein assay ~Peanuts~ 1,000,000 Relative Light Units (RLU) 100,000 10,000 ●： A3 1,000 発光量 ●： ATP 100 10 1 0.001 0.01 0.1 1 10 100 タンパク量 (μg/assay) Protein ( μg /assay) LOD of Bradford Protein assay ： 0.1 μg The sensitivity of A3 method for peanuts was equal to or better than that of protein assay. 19
A3 vs Protein assay ~Shrimp~ 1,000,000 Relative Light Units (RLU) 100,000 10,000 ●： A3 1,000 発光量 ●： ATP 100 10 1 0.01 0.1 1 10 100 Protein ( μg /assay) タンパク量 (μg/assay) LOD of Bradford Protein assay ： 0.1 μg The sensitivity of A3 method for shrimp was better than that of protein assay. 20
Chemicals in samplers ✓ Unwanted chemicals are contained in many products or on swabs, which can cause trace surface contamination, but A3 uses a dry swab that contains no trace contaminants Kikkoman 3M Hygiena Neogen Charm BioControl None (dry Chlorhexidinedi Benzethonium Does not No show (but Biofilm swab) gluconate Chloride contain any pre-moisten) breaking agent Humectant (SDS info) hazardous (leaflet info) (SDS info) substances at the concentration limits given in Regulation(EC) No.1272.2008 and OSHA HazCom Standard 29 CFR 1910.1200 (SDS info) 21
Conclusion ✓ ATP is easily decomposed into ADP/AMP Assays that rely on ATP alone can produce false negatives for surface cleanliness ✓ A3 assay (ATP+ADP+AMP) detects more – providing high sensitivity and more accurate sanitation verification ✓ LuciPac A3 is sensitive enough for allergen monitoring programs, incl. nuts and eggs ✓ A3 does not leave trace chemical contamination 22
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ATP and A3 detection in food products ✓ Conventional ATP tests do not detect many food products ✓ High Sensitivity LuciPac A3 shows a high level of detection Chicken Pork Sausage Tuna Shrimp 2500 4500 30000 4000 30000 4000 3500 25000 25000 2000 3500 3000 3000 20000 20000 2500 1500 Luminescence Intensity (RLU) 2500 15000 2000 15000 2000 1000 1500 1500 10000 10000 1000 1000 500 5000 5000 500 500 0 0 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 ATP A3 Squid Canned tuna Egg Peanut Dried mango 180000 60000 1200 18000 25000 160000 16000 50000 1000 20000 140000 14000 120000 40000 800 12000 15000 100000 10000 30000 600 80000 8000 10000 60000 20000 400 6000 40000 4000 5000 10000 200 20000 2000 0 0 0 0 0 ATP A3 ATP A3 ATP A3 ATP A3 ATP A3 24
ATP and A3 detection in beverages ✓ LuciPac A3 is more sensitive in beverage detection Luminescence Intensity (RLU) 25
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