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Z. Aspridou , M. Kakagianni, D. Dimakopoulou- Papazoglou, K. - PowerPoint PPT Presentation

Sep 25, 2022 •19 likes •220 views

Aristotle University of Thessaloniki Faculty of Agriculture, Forestry and Natural Environment School of Agriculture Laboratory of Food Microbiology and Hygiene Z. Aspridou , M. Kakagianni, D. Dimakopoulou- Papazoglou, K. Koutsoumanis 1 st

  1. Aristotle University of Thessaloniki Faculty of Agriculture, Forestry and Natural Environment School of Agriculture Laboratory of Food Microbiology and Hygiene Z. Aspridou , M. Kakagianni, D. Dimakopoulou- Papazoglou, K. Koutsoumanis 1 st Symposium on Space Educational Activities 9-12 December, Padova

  2. “Spin Your Thesis 2015” ESA campaign Microbiology and Hygiene Team (MAH) MAH Team Spin Your Thesis 2015

  3. Aristotle University of Thessaloniki Dep. Food Science and Technology Lab. Food Microbiology and Hygiene MAH Team Spin Your Thesis 2015

  4. Microbiology and Hygiene Team (MAH)  Microbiological quality and safety of fresh and processed food products Scientific Interests  Predictive microbiology  Quantitative microbial risk assessment Biofilms Motility Growth Hyper -gravity Inactivation limits Growth Resistance MAH team proposal MAH Team Spin Your Thesis 2015

  5. The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility Microbial resistance Microbial swimming motility  Heat  Microorganism specific  D value  Microscopic examination  Microorganism specific  Various types of motility  Invasion and virulence of the pathogens  Dispersal and colonization of niches  Sensitivity to other stresses  Competition phenomena  Biofilm formation  Virulence factor  Cross protection phenomena  Biofilm formation MAH Team Spin Your Thesis 2015

  6. The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility Microbial resistance  Heat  D value  Microorganism specific MAH Team Spin Your Thesis 2015

  7. The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility 10 9 8 Log CFU/ml 7 6 Spores of thermophiles 5 4 3 Vegetative cells 2 1 0 0 10 20 30 40 50 60 70 Time (min) MAH Team Spin Your Thesis 2015

  8. The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility Microbial swimming motility  Microorganism specific  Microscopic examination  Various types of motility  Dispersal and colonization of niches  Competition phenomena  Virulence factor  Biofilm formation MAH Team Spin Your Thesis 2015

  9. The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility MAH Team Spin Your Thesis 2015

  10. Objective 1: Heat resistance  Salmonella enterica ser. Agona  Geobacillus stearothermophilus Type of microorganism Temperature Gravity 57 and 65 ° C Mesophilic (planktonic) 10xg, 20xg 121 ° C Thermophilic (spores) 20xg Survival data Initial population : N 0 Final population : N residual MAH Team Spin Your Thesis 2015

  11. Objective 2: Motility  Pseudomonas fluorescens Type of microorganism Temperature Gravity Mesophilic (planktonic) ambient 1-20xg Time lapse videos Cells’ speed, displacement distributions MAH Team Spin Your Thesis 2015

  12. Objective 1 Need for consumables  Organization Shipment  Collaboration Proper storage Need for equipment Objective 2 Time limitation Experimental set up Check of the system functionality Sample preparation inoculum and integrity Time limitation MAH Team Spin Your Thesis 2015

  13. Objective 1: Heat resistance Difference in the survival under various g levels Different behavior of the survivors Sequential exposure - Gravity to Heat stress Low to moderate hyper-gravity levels as a stress MAH Team Spin Your Thesis 2015

  14. Objective 2: Motility 2g 1g MAH Team Spin Your Thesis 2015

  15. Objective 2: Motility 2g sequential 8g MAH Team Spin Your Thesis 2015

  16. Objective 2: Motility  Speed  Tumbling  Direction  Runs  Step  Energy ImageProPlus MAH Team Spin Your Thesis 2015

  17. Low to moderate levels of hyper-gravity • Stress for the microorganisms • Microbial response mechanisms • Microbial energy  Explanation of microbial behavior  Design new processes (Disinfection)  Selection of microorganisms- Technological and functional properties  Terrestrial and extraterrestrial application MAH Team Spin Your Thesis 2015

  18. MAH Team – Spin Your Thesis 2015 MAH Team Spin Your Thesis 2015

  19. Acknowledgements European Space Agency Education Office and Aristotle University of Thessaloniki for their support European Space Agency Education Office for the scholarship Members of the ESA LDC and LIS laboratory for their help MAH Team Spin Your Thesis 2015

  20. Aristotle University of Thessaloniki Faculty of Agriculture, Forestry and Natural Environment School of Agriculture Laboratory of Food Microbiology and Hygiene Thank you for your attention Z. Aspridou , M. Kakagianni, D. Dimakopoulou- Papazoglou, K. Koutsoumanis 1 st Symposium on Space Educational Activities 9-12 December, Padova

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