THE SCIENCE OF NOVACQ TM IN PENAEUS MONODON Ha Truong, Tansyn Noble - PowerPoint PPT Presentation

Thailand 14 active Novacq ponds Australia - Yamba active Novacq ponds THE SCIENCE OF NOVACQ TM IN PENAEUS MONODON Ha Truong, Tansyn Noble and Omar Mendoza-Porras 31 st July 2019 | APFA Brisbane Convention and Exhibition Centre SUSTAINABLE AQUACULTURE PRODUCTION Ridley and CSIRO alliance

Outline Ridley and CSIRO alliance Novacq and other marine bioactives on: 1. Growth under different feeding regimes 2. Gut microbiome 3. Health markers 2 | Science behind Novacq TM

Ridley and CSIRO science alliance Three post-docs focusing on • Nutrition • Immunity/health • Proteomics Novacq TM : A novel aquafeed additive that uses natural microbial processes to increase growth, feed efficiency and health, and reduce reliance on fishmeal • Relatively low in protein and high in carbohydrate and ash • Greater than 30% increase in weight gain in monodon in tanks 3 | Science behind Novacq TM

Marine ingredients Novacq TM Krill, squid, prawn meal • Strategic ingredients in prawn • Source of microalgae, yeast, feed bacteria, living and dead organic matter, carbon, minerals • Improves growth, intake and feed • Improves growth, feed efficiency conversion efficiency and survival Mechanisms? Feed attractant Complete source of micronutrients Unknown bioactives Nutrient utilisation Meeting a deficiency 4 | Science behind Novacq TM

Communal growth trial Dietary treatment Krill g/kg Fishmeal Krill meal hydrolysates Novacq ™ Fishmeal 300 210 210 300 • 10 prawns/tank; 4 tanks Novacq 100 Krill meal 100 reps/dietary treatment Krill • Iso-nitrogenous and energetic hydrolysate 100 Soybean 300 300 300 300 diets offered to communally- Gluten 70 70 70 70 housed juvenile black tiger prawns Wheat flour 295 285 285 195 for 6 weeks Fish oil 15 11 11 16 Micro- 19 ingredients 19 19 19 Fed at satiation (120-140%) or restricted (70%) 5 | Science behind Novacq TM

Growth under different feeding regimes Ha

Growth results Weight gain (g) 9 8 7 86% 6 5 4 3 2 1 0 Control Krillmeal Krill NovacqTM Control Krillmeal Krill NovacqTM hydrolysate hydrolysate Satiation Restricted Novacq TM bioactivity was enhanced when unrestricted-fed 7 | Growth effect of marine bioactives | Dr Ha Truong

Growth results FCR (feed:gain) 3.5 3 2.5 2 1.5 38% 1 0.5 0 Control Krillmeal Krill NovacqTM Control Krillmeal Krill NovacqTM hydrolysate hydrolysate Satiation Restricted Novacq TM obtained the best FCR • • Restricted-fed diets were more efficient but growth was poor 8 | Growth effect of marine bioactives | Dr Ha Truong

Other parameters measured: • Nutrient retention • Apparent nutrient digestibility • Protease and amylase activity • Intake rate • Gut evacuation rate • Behavioural attraction assessment 9 | Growth effect of marine bioactives | Dr Ha Truong

Gut microbiome Tansyn

Why look at the microbiome? • Microbiome linked with numerous health conditions. • Right balance between beneficial and non- beneficial bacteria. • Diet influences microbiome • Know diets especially Novacq improves growth and nutrient efficiency in prawns. • Is this improved performance linked with having a unique microbiome? • Used DNA sequencing to identify the bacterial community present. 11 | Presentation title | Presenter name

Prawn gut microbial profile • • Highly diverse with 1435 different bacteria Broad-scale clustering based on similarity types found • No clustering of samples due to diet • Diets have similar bacterial composition • Clear separation between the two feeding • Varying levels of vibrios regimes 12 | Microbiome changes to feed | Dr Tansyn Noble

A closer look at some taxa Both feeding regime and diet significantly influence specific taxa abundance. Abundance Diet 13 | Microbiome changes to feed | Dr Tansyn Noble

Haemolymph health markers Omar

Advanced understanding of prawn health and immunity using proteomics Antioxidant Oxygen Capacity Transport Innate • Immunity Simultaneous quantification of multiple target proteins currently 50 and increasing • Application across various Stress tissues Growth Response and metabolism • Non-destructive blood sample Reproduction and Development • Functionally relevant to prawn biology Evaluate the effect of diet, environmental or pathogen challenge 15 | Developing blood health markers by proteomics | Dr Omar Mendoza-Porras

Broad effects of Novacq Unrestricted fed cohort Control Novacq Novacq Novacq Novacq Control Consistent separation of dietary treatments 16 | Developing blood health markers by proteomics | Dr Omar Mendoza-Porras

Expression of health markers a 0 .6 0 .4 b b 0 .2 0 .0 Control a 0 .6 Novacq 0 .4 a Novacq b 0 .2 0 .0 0 .2 5 a Higher expression means that 0 .2 0 homeostasis is not optimal. Energy 0 .1 5 a a potentially diverted into metabolic 0 .1 0 adjustments rather than growth. 0 .0 5 0 .0 0 17 | Developing blood health markers by proteomics | Dr Omar Mendoza-Porras

THANK YOU ANY QUESTIONS? Special thanks Agriculture and Food Ha Truong Ridley Post doctorate fellow • Dr Matt Briggs e ha.truong@csiro.au • Dr Viviana Lazzarotto Tansyn Noble Post doctorate fellow CSIRO e tansyn.noble@csiro.au • Dr Cedric Simon • Dr Nick Wade Omar Mendoza Porras • Dr James Wynne Post doctorate fellow • Dr Artur Rombenso e omar.mendozaporras @csiro.au • BIRC and QBP staff AGRICULTURE & FOOD