SCHOTT Lighting and Imaging SCHOTT Microscopy www.lambdaphoto.co.uk T: +44 (0)1582 764334 LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Contents Contents • overview general lighting situations • illumination with fiber optics • direct LED illumination LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Microscope Types Types Microscope Classic (Light) Microscope Stereo Microscope LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Types of Stereo of Stereo Microscopes Microscopes Types „GREENOUGH“-type „CMO“-type ( C ommon M ain O bjectíve) LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Contrasting of specimen in stereo - microscopy is highly dependent on ... the choice of the right illumination system!!! LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Overview Illumination Illumination Techniques Techniques Overview INCIDENT LIGHTING TRANSMITTED LIGHTING INCIDENT LIGHTING TRANSMITTED LIGHTING • BRIGHTFIELD • BRIGHTFIELD illumination oblique illumination • DARKFIELD illumination (spotlight, lightline) - polarization • BRIGHTFIELD homogeneous illumination (ringlight) • DARKFIELD illumination - polarization - fluorescent illumination - co-axial illumination - near vertical illumination LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Incident Light, Light, Ringlight Ringlight - - Homogeneous Homogeneous Illumination Illumination Incident THEOBROMA CACAO, Cacao bloomer LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Incident Light, Light, Gooseneck Gooseneck ( (Shadowing Shadowing) ) Incident EUPHOLUS LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Incident Ringlight Ringlight vs vs Oblique Oblique Light Line Light Line Incident ringlight corrosion oblique light line LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Incident Light, Light, Darkfield Darkfield Ringlight Ringlight Incident EURO coin LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Incident Ringlight Ringlight vs vs Darkfield Darkfield Ringlight Ringlight Incident ringlight ringlight darkfield LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Incident Ringlight, Ringlight, Polarization Polarization Incident without polarization analyzer polarizer polarization LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Incident Ringlight, Ringlight, Polarization Polarization Incident without polarization analyzer polarizer polarization LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Incident Fluorescent Fluorescent Lighting Lighting Incident without cut-off filter Cut-off filter excitation Cracks at solder joint LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Coaxial Incident Incident Lighting Lighting Coaxial polished stone LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Near Vertical Vertical Illumination, Illumination, Inspection Inspection of of Drilled Drilled Holes Holes Near valve LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Backlight, , Brightfield Brightfield Backlight bat embryo LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Backlight, , Darkfield Darkfield Backlight section, rat embryo LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Backlight, , Polarization Polarization Backlight Nylon fibre analyzer polarizer Cotton fibre LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Contents Contents • overview general lighting situations • illumination with fiber optics • direct LED illumination LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Illumination Systems with with fiber fiber optics optics Illumination Systems KL Series with halogene bulb KL Series with LED light engine LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Major Application Areas Major Application Areas • Stereo-Microscopy • Medical Technology Material Science • Laboratory (Industrial) • Machine Vision Life Science LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Halogene Light Sources with Fiber Optics Light Sources with Fiber Optics Halogene KL 300 KL 2500 LCD KL 1500 compact KL 1500 LCD LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging LED Light Sources with Fiber Optics LED Light Sources with Fiber Optics KL 2500 LED KL 300 LED KL 1500 LED KL 1500 LED plus NEW LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Spectrum of White LEDs LEDs Spectrum of White yellow Phosphor emission combined spectrum of white LED Spectrum blue InGaN LED-Chip 400 470 550 700 LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Emission Spectrum of KL 1500 LED vs. KL 1500 LCD Emission Spectrum of KL 1500 LED vs. KL 1500 LCD 3,0E+04 KL 1500 LED 2,5E+04 KL 1500 LCD 2,0E+04 intensity 1,5E+04 1,0E+04 5,0E+03 0,0E+00 350 400 450 500 550 600 650 700 750 800 wavelength [nm] LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging General Advantages of LED Light Sources General Advantages of LED Light Sources • white light (daylight, 5600-6000 K) • nearly constant color temperature on dimming • high reliability of LEDs (50,000 operating hours) • reduced „cost of ownership“ (no bulb changing) • low energy consumption (~ 20% compared to halogene) • wide range input (world wide selling) • DC output (no flickering) LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Major Advantages of LED Light Sources Major Advantages of LED Light Sources Cost comparison KL1500 LCD vs KL1500 LED essential reduction in weeks / year 52 days / week 5 „cost of ownership“ hrs / shift 8 shifts / day 2 hrs / year 4.160 LED lifetime / hours 50.000 LED lifetime / years 12,02 Bulb lifetime / hours 200 • LED lifetime 50,000 h Bulb lifetime / months 0,58 LED wattage / Watt 40 (70% reduced in brightness) Hal. wattage / Watt 200 Price of bulb / € 20 Price of kWh Energy / € 0,20 - no bulb on stock Savings lifetime per year - no service or downtimes Nr of bulbs 250,0 20,8 Cost of bulbs / € 5.000 416 Energy / kWh 8.000 666 Energy cost / € 1.600 133 • low energy consumption Total / € 6.600 549 - approx. 80% of energy savings Cost of lightsource / € 599 Payback time / years 1,09 (40 W vs 200 W) LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Advantages of LED Light Sources with Fiber Optics Advantages of LED Light Sources with Fiber Optics • significantly higher light intensities can be focussed to a small field of view • get more light out of given LED technology ⇒ (separate housing - more efficient cooling - bigger refelectors - more sophisticated optics) • installed base of light guides can be used (just change the light source) ⇒ full flexibility for realizing different illumination techniques • no bulky light source housing covers view to specimen • compact housing somewhere behind the microscope LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging Advantages of LED Light Sources with Fiber Optics Advantages of LED Light Sources with Fiber Optics LED Ringlight VisiLED S80-55 at 75 mm working distance compared to gooseneck light guide with KL 1500 LED LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging SCHOTT KL Series Illumination for Stereo Microscopy KL 300 LED LED light source with single LED light engine • brightness equivalent to 30 W halogene light sources • usable as standalone or mounted at microscope stands • fits to all light guides and accessories of KL 300 series • wide range power supply with international clip system • no cooling fan (free of noise and vibration) • continuous electronical dimming 0...100% • separate on/off switch • DC driven • nearly no color temperature shift on dimming LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging SCHOTT KL Series Illumination for Stereo Microscopy KL 300 LED overview mounting adapters mounting at microscope stand using as standalone LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging SCHOTT KL Series Illumination for Stereo Microscopy KL 1500 LED KL 1500 LED LED light source with multiple LED light engine • brightness equivalent to 150 W halogene light sources • fits to all light guides and accessories of KL 1500 series • inexpensive adapters for other systems available • slider for color filters • wide range power supply with international clip system • very low noise cooling fan • continuous electronical dimming 0...100% • separate on/off switch • DC driven • nearly no color temperature shift on dimming LE-RS/PSb, 24.05.2011
SCHOTT Lighting and Imaging SCHOTT KL Series Illumination for Stereo Microscopy KL 1500 LED plus KL 1500 LED plus LED light source with multiple LED light engine • basic features like KL 1500 LED • light setting linear controllable in 0.1%, 1% and 5% steps • display, which indicates brightness setting, status, or error codes • remote controllable with PC (via USB), foot switch or external remote control • electronic shutter function • flash function LE-RS/PSb, 24.05.2011
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