PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 Courtney Phillips Wednesday, March 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 1
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 ������������ ��������� ������� Federal ��������� FDCA Food, Drugs & Cosmetics �������������� Federal Act Insecticide, ���������� FIFRA Fungicide & Rodenticide ���������� Act For more information, contact courtney.phillips@perkinelmer.com 2
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 3
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 4
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 SPE ����������� Quechers Dilution Automated variants ���������� Liquid Chromatography* ��������� MS/MS High Resolution Mass Analyzer SPE ����������� Quechers Solid Phase Extraction Separates analytes of interest Dilution from a complex matrix Phases similar to LC columns Automated variants Requires vacuum manifold ~ 5 – 10 min/sample ���������� Liquid Chromatography* ��������� MS/MS High Resolution Mass Analyzer For more information, contact courtney.phillips@perkinelmer.com 5
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 SPE ����������� Quechers Dilution Quick, Easy, Cheap, Effective, Rugged & Safe Developed specifically for Automated variants pesticides analysis in foods Created as an alternative to the Luke method Requires centrifuge ~ 5 – 10 min/sample ���������� Liquid Chromatography* ��������� MS/MS High Resolution Mass Analyzer SPE ����������� Quechers Dilution Automated variants ���������� Liquid Chromatography* Considerations for Liquid Chromatography Mass analyzers require lower flow rates UHPLCs have the ability to run at these flow rates ��������� MS/MS However, traditional UHPLC columns may not be ideal – Consider Superficially Porous Particle (SPP) columns High Resolution Mass Analyzer For more information, contact courtney.phillips@perkinelmer.com 6
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 SPE ����������� Quechers Dilution Automated variants Triple Quadruple Instrument ���������� Liquid Chromatography* ��������� MS/MS High Resolution Mass Analyzer SPE ����������� Quechers Dilution Automated variants Time of Flight (TOF) Instrument ���������� Liquid Chromatography* ��������� MS/MS High Resolution Mass Analyzer For more information, contact courtney.phillips@perkinelmer.com 7
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 LC-TOF Pesticides by LC-TOF Prometryne/dichloran Diazinon Acetamiprid Malathion/metalochlor Mevinphos isomers Mevinphos Oxamyl Dimethoate Acephate Spinosad A Methomyl Anilazine For more information, contact courtney.phillips@perkinelmer.com 8
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 Pesticides by LC-TOF Pesticides by LC-TOF ������������������������������������������������ For more information, contact courtney.phillips@perkinelmer.com 9
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 Spiked Pesticides in Celery Extraction by Quechers RP-LC Pos ion mode Dual probe ESI- TOF with lock mass calibration Carbendazim in Orange Juice RP-HPLC Brownlee C18 SPP 10cm m/z 100-400 Dual ESI Source with Lock Mass Gradient from 30% � � 100% MeOH � � 1.5 spectra/sec Drying Gas Flow 12 L/min Ambient temp, 0.3mL/min Flow Drying Gas Temp 350 o C Capillary exit voltage = 90 V For more information, contact courtney.phillips@perkinelmer.com 10
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 Carbendazim in Orange Juice RP-HPLC Brownlee C18 SPP 10cm m/z 100-400 Dual ESI Source with Lock Mass Gradient from 30% � � � � 100% MeOH 1.5 spectra/sec Drying Gas Flow 12 L/min Ambient temp, 0.3mL/min Flow Drying Gas Temp 350 o C Capillary exit voltage = 90 V Carbendazim in Orange Juice RP-HPLC Brownlee C18 SPP 10cm m/z 100-400 Dual ESI Source with Lock Mass Gradient from 30% � � � 100% MeOH � 1.5 spectra/sec Drying Gas Flow 12 L/min Ambient temp, 0.3mL/min Flow Drying Gas Temp 350 o C Capillary exit voltage = 90 V For more information, contact courtney.phillips@perkinelmer.com 11
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 Carbendazim in Orange Juice All data, all the time… For more information, contact courtney.phillips@perkinelmer.com 12
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 All data, all the time… All data, all the time… For more information, contact courtney.phillips@perkinelmer.com 13
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 LC-TOF ��������� �!�!"# For more information, contact courtney.phillips@perkinelmer.com 14
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 15
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 16
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 17
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 18
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 ����$�������%&������&'��������(��)��*�"�����+&���� ������������������� ����'�������������� , �������-������.� �����������&'�� For more information, contact courtney.phillips@perkinelmer.com 19
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 /&����������� ��0&���������������1 ��0&��� �)������$���)� ������������0&����� ����������'�2��� �&�)����������'�� 0&���������������� �'�$)�������&���� For more information, contact courtney.phillips@perkinelmer.com 20
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 ��������$�.� 3������������������ �����)��4���(��)��� ��������� ����������)���&�&��1� ��''���&��������� 5��(�����6 For more information, contact courtney.phillips@perkinelmer.com 21
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 /&�������6 For more information, contact courtney.phillips@perkinelmer.com 22
Recommend
More recommend