Natural Protection of Milk 14 th April 2016 Host Institution - PowerPoint PPT Presentation

Natural Protection of Milk 14 th April 2016 Host Institution

Allergy prevalence • Allergic disease major escalating health issue • Atopic dermatitis, food allergy, asthma • 2 - 3% infants in 1 st year – cow milk allergy • 5% children develop food allergies • Majority ‘grow out’ of the problem • Persistent sensitization = increased risk of later allergy

The Allergic March Typical age of onset https://www.allergyuk.org/causes-and-risks-of-allergy/the-allergic-march

Epidemiological studies • Studies in Europe and New Zealand show a relationship between farms and allergy • Farming families have a decreased risk of developing allergy Braun-Fahrlander et al (1999), Ernst and Cormier (2000), Kilpelainen et al (2000), Riedler at al (2000), Wickens et al (2002)

Protection from allergy • Exposure to barns and stables • Early contact with farm animals • Working on a farm during pregnancy • Consumption of unpasteurised milk Loss et al (2011), van Neerven at al (2012), von Mutius and Vercelli (2016)

What’s in milk? • Fat, protein, carbohydrates • Complex mixture • Characteristics altered by processing • Antibodies • Hormones proteins • Enzymes whey proteins 30.0 g/l • Growth factors 6.0 g/l • Cytokines other 1.5 g/l • Nucleotides a-lact 1.5 g/l • Polyamines caseins b-lact 3.0 g/l • Free amino acids 24.0 g/l • Free fatty acids • monoglycerides • MFGM proteins • Oligosaccharides

Can milk affect allergy responses? Mouse model for food effects on allergy (Brandt et al 2003) 0 14 28 30 32 34 days IP saline or OVA antigen Saline or OVA Sensitisation x2 Intra-gastric challenges OVA = ovalbumin protein, IP= intraperitoneal injection Diet: Raw milk Sterilised milk ( γ irradiated) Heated milk

Effects of milk treatment Sterilised milk Heated milk Raw milk Gamma Heat to 87°C irradiated and cooled

Effects of milk treatment

Yes, milk affects allergy responses c 15000 c 10000 MMCP-I (ng/ml) IL-10 (pg/ml) b 10000 7500 b b b b 5000 b 5000 2500 a a 0 0 Control Water Raw Sterilised Heated Control Water Raw Heated Gamma OVA treated OVA (Hodgkinson et al 2014)

Natural protection of milk Hypothesis : A safe milk product, produced using special processing techniques to preserve heat-sensitive components, will retain health benefits associated with unprocessed milk. Research Plan : • Models to demonstrate protective effects • Mechanism of action and potential biomarkers • Timing and duration of dose

Natural Protection of Milk • Unlock the natural health benefits of milk • In partnership with Miraka, develop a safe product that delivers added health benefits to toddlers • Meet a demand from parents for natural foods to reduce risk allergy development in their children