Malaysian Healthy Ageing Society Badriya Al-Rahbi 1 Sangu Muthuraju, - PowerPoint PPT Presentation

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Badriya Al-Rahbi 1 Sangu Muthuraju, 2, Rahimah Zakaria 1 , Zahiruddin Othman 3 , Asma ’ Hassan 4 1 Department of Physiology, 2 Department of Neuroscience, 3 Department of Psychiatry , 4 Department of Anatomy School of Medical Sciences, USM

 Emotional disorders show sex differences in prevalence, with women having a higher tendency to develop anxiety or depression (Breslau et al., 1999 & Aitken and Baker, 2006).  Women have a higher probability of developing ma majo jor r anxiety when they are 45 – 55 years old than at other ages. (Boivin and Schmidt, 2005; Schmidt et al., 2005)

 Estrogen was found to induce anxiolytic effects through its antioxidant property (Frye and Wawrzycki, 2003; Hill et al., 2007; Nomikos and Spyraki, 1988; Walf and Frye, 2005).  However, many postmenopausal women refused to take HRT because of its side effects e.g. en endome metrial trial blee eedi ding ng . -There is a need to find an alternat rnative ive treatm eatmen ent to HR HRT. T.

 Therefore, this study aims: to eva valuat uate e possib sible e anxiolyt lytic ic effect t of Tualang ang honey ey in ovx vx rats s exposed ed to social stress ess and to compare the effects with E2 treated ovx rats exposed to social stress.

Femal ale and male Sprague ue Dawley rats 180-200 g (age 8 weeks ) n=10 Operated ed u under adequate e anaesthesia esia : (keta etamine mine 90 90mg mg / kg kg & & xylazi zine ne 10 10mg/kg kg (IP) P) Femal ale rats were sham Femal ale e rats s were e bilateral ally y operate ted ovariecto ctomi mize zed

General anesthesia operation ovx) ) Post operative care (10 days) Recovery period 60 days

After 2 months of recovery period. The rats were divided into six groups (n=10) Group 2: Group 3: Group 4: Group 5: Group 6: Group 1: OVX + No stress OVX +Stress OVX + stress + 17 β - OVX +stress + honey Sham-operated Sham Control+ estradiol (s.c), 20  g (orally), 0.2g/kg . Control + stress No stress Treatment with either 17  -estradiol or Tualang honey were started from 3 3 days before the stress procedur ure.

Stre ress s pro rocedu cedure re

Stress procedur ure Social instability crowdi cr wding ng isolation ation phase ses s phase ses s (24 24h) h) (24 24h) h) For 15 days

Open en Field test st (OFT) ) : is a common behavi viour ural al test for examine anxiety in rodents Main behavio viour urs sco core red: d:  Rearing number..  Grooming Time..  Freezing Time..  Time spent crossing the lines..  Defecation …

Biochemic ochemical al Test

Stress procedure behav avioura oural assessment (OFT) Animal sacrificed, blood collected for estimation of serum estradiol and corticosterone levels E & CORT were determined ELISA kits (Creative Diagnostic, USA) statistical analysis

 The data were expressed as the mean ± SEM.  Main anxiety behaviours were subjected to a two-way analysi sis s of variance having two levels of surgery (ovx, non-ovx) and two levels of stress (no stress, stress).  One-way (ANOVA).  All effects were statistically significant at the p< 0.05.

 The main effect t of surge gery ry yielded an F ratio of F (1, 36) = 69.06, p < .001  indicating that the mean change of anxiety behaviours was significantly greater for ovx ( M =7.09 ?, SD = 1.21) than for non-ovx (M=4.60 , SD =0.82)  The main effect t of stress ess yielded an F ratio of F (1, 36) =3.29, p < .01  indicating that the mean change of anxiety behaviours was significantly higher in the stress (M =6.02, SD = 1.14) than in the non-stress (M =5.19 , SD = 0.92).

 a significant interaction effect between the Stress ess * s * surgery gery (ovx vx) ) on total rearing number F (1, 36) =11.16, p< p<0.05 05). ).

#P<0.01 ovx + stress+ E / H compared to ovx +St

Grooming Time… *P<0.05 ovx+ stress+ E / H compared to ovx +St

 low estrogen in ovx  Compar pared ed to co control rol groups oups  $P $P<0.001 001  high CORT in stress s animals als  Stress ss ovx vs. non stressed sed cont groups… #P< 0.01 01

 Th The levels s of COR ORT T were higher r in stress ss than non- stress but E2 treatment and TH supplement rats showed lower levels of CORT compared to the untreated ovx rats with stress  This indicates that both Estrogen treatment / Honey supplementation were able to reduce ce the stress s level co compar mparabl able e to non-stre stress ss rat ats

 In conclusion, this study showed that Tualang honey supplement to ovx rats with stress reduced the anxiety behaviours (i.e grooming time.) compared to its untreated counterparts. The effects were comparable to the E2 treated ovx rats with stress.  Tu Tualang ng honey y co could d potentia ntially lly be used as an alternative ative therapeuti apeutic c agent t against st anxiety ty symptom toms s in menopausal opausal women. n.

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