M. Kyriakou 1 , V. Chatziiona 1 , C. Costa 1 , L. Koutsokeras 2 , G. - - PowerPoint PPT Presentation

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M. Kyriakou 1 , V. Chatziiona 1 , C. Costa 1 , L. Koutsokeras 2 , G. - - PowerPoint PPT Presentation

Nov 07, 2023 441 likes •658 views

Environmental Bioprocessing Laboratory M. Kyriakou 1 , V. Chatziiona 1 , C. Costa 1 , L. Koutsokeras 2 , G. Constantinides 2 , M. Koutinas 1 1 Department of Environmental Science & Technology, Cyprus University of Technology 2 Department of

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  • M. Kyriakou1, V. Chatziiona1, C. Costa1, L. Koutsokeras2,
  • G. Constantinides2, M. Koutinas1

Environmental Bioprocessing Laboratory

1 Department of Environmental Science & Technology, Cyprus University of Technology 2 Department of Mechanical Engineering and Materials Science and Engineering, Cyprus University of Technology

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 Carbon‐rich material  Produced from biomass (such as wood, manure or leaves)

through the pyrolysis process

 Thermal decomposition of organic material under limited

supply of oxygen (O2), and at low temperatures (<700 °C)

Pyrolysis Solid ‐ Biochar Liquid ‐ Bio oil Gas ‐ Syngas

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 Environmental remediation  Soil amendment  Removal of organic pollutants  Mitigate climate change

  • Industrial interest where it could be

employed as a renewable and low‐cost support material

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Attachment Aggregation Entrapment Containment

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 High biomass concentration  Easy separation of the biomass from the liquid  Increased product yield  Increased product stability  Imparts a special stability to the microorganism against

environmental stresses

 Protects cell from shear forces

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 Produce biochar from different feedstock  Study the industrial application of biochar  Economical substitute to the activated carbon  Bioethanol production

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Biomass sources Pyrolysis 250 ᵒC and 500 ᵒC

Biochar Ethanol Succinic acid Immobilization Char of non biological origin Material characterization

Olive kernels Sludge Vineyard prunings Seaweed

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  • Porosity
  • Structure
  • Surface characteristics

SEM

  • Elemental analysis
  • Composition &

percentage

EDX

  • Phase identification of a

crystalline material

  • Information on unit cell

dimensions

  • Specific surface area

XRD BET

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Halite (NaCl) Calcite (CaC03), Silicone (Si)

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Mag: 3000x

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Non‐biological char

Mag: 3000x

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Olive kernels 250 ᵒC 500 ᵒC Vineyard prunings 250 ᵒC 500 ᵒC

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Sludge 250 ᵒC 500 ᵒC Seaweed Non‐biological char

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Samples Temperature (ᵒC) BET specific surface area (m2g‐1) Olive kernels 250 0.1479 500 1.5181 Vineyard prunings 250 0.4701 500 41.7288 Sludge 250 0.6914 500 1.3559 Seaweed 250 1.9143 500 5.3247 Non‐biological char ‐ 72.9874

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Vineyard prunings Seaweed Non‐biological char Immobilized cells Free cells

Bioethanol production

Saccharomyces cerevisiae Kluyveromyces marxianus Pichia kudriavzevii KVMP 10

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Liquid media simulating Valencia orange peel waste hydrolysate that consisted of (g/l):

  • Fructose

33.2

  • Galactose

8.6

  • Glucose

57.4

  • Sucrose

1.4 Saccharomyces cerevisiae Kluyveromyces marxianus Pichia kudriavzevii KVMP 10

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10 20 30 40 50 60 70 80 90 3 6 9 12 15 18 21 24 27 30 33

Ethanol (g/l) Time (h)

Saccharomyces cerevisiae

Liquid media simulating Valencia orange peel waste hydrolysate that consisted of (g/l):

  • Fructose

33.2

  • Galactose

8.6

  • Glucose

57.4

  • Sucrose

1.4 Vineyard prunings biochar 77 g/l Seaweed biochar 70 g/l Free suspended cells 68 g/l

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  • Pyrolysis temperature
  • Physicochemical characteristics
  • Potential use
  • Bioethanol production using freely suspended cells
  • S. cerevisiae produced 51 g/l bioethanol at 37 ᵒC
  • K. marxianus produced 45 g/l bioethanol at 42 ᵒC
  • P. kudriavzevii produced 44 g/l bioethanol at 42 ᵒC
  • Bioethanol production using immobilized cells of S. cerevisiae
  • Vineyard prunings biochar 77 g/l
  • Seaweed biochar

70 g/l

  • Free cells of S. cerevisiae 68 g/l

500 ᵒC

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Substrate Support material Yeast Initial sugar concentration (g/l) Ethanol production (g/l) Literature

FREE CELLS

Valencia orange peel model solution ‐

  • P. kudriavzevii

KVMP 10 101.0 54.0 Koutinas et al., 2015 Valencia orange peel model solution ‐

  • K. marxianus

101.0 37.1 Wilkins et al., 2007 Valencia orange peel model solution ‐

  • S. cerevisiae

101.0 40.9 Wilkins et al., 2007 Valencia orange peel model solution ‐

  • S. cerevisiae

101.0 40.0‐45.0 Grohmann et al., 1994 Valencia orange peel model solution ‐

  • P. kudriavzevii

KVMP 10 101.0 44.0 Current study Valencia orange peel model solution ‐

  • K. marxianus

101.0 45.0 Current study Valencia orange peel model solution ‐

  • S. cerevisiae

101.0 51.0 Current study

IMMOBILIZED CELLS

Glucose Sodium alginate

  • S. cerevisiae

50.0 52.0 Najafpour et al., 2004 Beet molasses Sodium alginate

  • S. cerevisiae

250.0 53.0 Roukas 1996 Glucose γ‐alumina

  • S. cerevisiae

113.0 80.0 Kana et al., 1989 Valencia orange peel model solution Vineyard prunings biochar

  • S. cerevisiae

101.0 77.0 Current study Valencia orange peel model solution Seaweed biochar

  • S. cerevisiae

101.0 70.0 Current study

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 Immobilization experiments for bioethanol production

using Pichia kudriavzevii and Kluyveromyces marxianus

 Immobilization experiments for succinic acid production  Comparison of biochar with other support materials

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