IMAGING SCIENCE SPOTLIGHT SERIES February 3 rd , 2016 Texas A&M - PowerPoint PPT Presentation

IMAGING SCIENCE SPOTLIGHT SERIES February 3 rd , 2016

Texas A&M Institute for Preclinical Studies Joseph Kornegay Director Operating Rooms PET/CT Micro-PET/ Additional Modalities: Radio-pharmacy 3 large state-of-the-art OR Siemens Biograph SPECT-CT Ultrasound Radiochemistry Suites Fixed Cardiac mCT 128-slice TOF Alpbira Bruker Planar X-ray Laboratory Catheterization Lab PK/PD Biospin Corp. Portable C-Arm In-House sterilization (ETO Radiochemistry and PACS and Steam) Labeling Facilities Autoradiography techniques Using multiple beta and Auger emitters 2

The Center for Transla.onal Research in Aging and Longevity (CTRAL) is engaged in ongoing TranslaAonal research in humans in the fields of nutriAon, exercise, and metabolism in relaAon to aging and the common diseases and condiAons present in our aging populaAon. This includes Cancer, Chronic Heart Failure, Chronic Obstruc.ve Pulmonary Disease, Demen.a and mild cogni.ve impairment (MCI), Au.sm, Obesity, Sleep apnea, and Inac.vity/Bed rest. Used research approaches comprise intravenous infusions of stable isotopes to measure metabolism (i.e., energy, protein, amino acids, fat), quan.fica.on of muscle mass, and func.on tes.ng of muscle, liver and brain. Typical Research Ques.ons 1. Does leg exercise in older adults with a chronic disease improve muscle energy kine.cs by training/nutri.on ( 31 P- 1 H- 13 C-MR; figure )? 2. Is obesity associated with changes in Intra/Extra cellular muscle/liver fat content? 3. To what extent does bed rest reduce whole body muscle content? 4. Is OSA (Obstruc.ve Sleep Apnea) associated with altera.ons in brain func.on and ac.vity? Boesch 2007 5. fMRI and aging and chronic diseases PRIORITY 1. NMRS: 31 P, 1 H, 13 C 2. Body composition/imaging 3. fMRI

3D imaging of fossil pro.st/algal shells, cysts and skeletal components John V. Firth, Curator, IODP-TAMU Fossils Method Results Discovered NEED! Short, medium Micro-CT 1) Visible laser (488 (best resoluAon nm best) fluoresce +/- long UV =0.7 µm/700 nm) calcite microfossils, (220-360 nm) though UV light is laser/LED supposed to produce source for best calcite fluorescence confocal 2) Visible laser (488 microscopy nm best) fluoresces to get the opaline silica 3D hand size strongest microfossils print of foram (radiolarians, diatoms, fluorescence silicoflagellates, etc.) NEED!! Nano-CT (50 and best 3D 700 µm nm resoluAon) such 3) Variable images of the Cylinder with fluorescence in as Xradia 800 or radiolarian tests Aniest 3D hand size siliceous fossils (opaline silica) & Bruker SkyScan 2211 microfossils print of rad reflects varia.ons in foraminifer shells Nanotomograph trace elements within (calcite) (63-400 µm) single specimens Calcareous Nannofossils Diatoms Method Confocal Laser 3D hand 3D hand Fluorescence size size print of (best resoluAon print of nannofossil = ~70 nm) diatom Size = 4-20 µm Size = 10-200 µm

Sensors for the fluorescence turn-on detec@on of F - and CN - in water New radiotracers for positron emission tomography (PET) PET/Fluorescence dual modality imaging agents Francois Gabbaï – Chemistry – hFp://www.chem.tamu.edu/rgroup/gabbai/Publica@ons.html

TAMU Vet. Med. Teaching Hospital • Pets are naturally occurring models of human disease! • Broad diversity of experts (vet radiologists, neurologists, surgeons, anesthesiologists, … • Research – Quantitative MRI – Technique validation • Capabilities – 3T MRI, 40-slice CT, ultrasound, nuc med • Collaborators – Siemens, MD Anderson Diffusion Tensor Tractography (Dachshund)

Confocal Imaging for Biomedical Application of Synthetic Polymers Soon Mi Lim / Karen L. Wooley, Dept. of Chemistry, Texas A&M University Bad cells Cytoskeleton Good cells Degradable polycarbonate for bone implant Preosteoblast cells w/ degradation Nuclei on 1,2 EDT- co -TAQA compounds for 72hr Polymer substrate Focal adhesion PEGylated polyphosphoester-based nanocarriers for improving paclitaxel delivery for cancer treatment Lauren Link 10 µ m 100 µ m poly-Honokiol for cardiovascular application Endothelical cells on poly-Honokiol, cytoskeleton (green) and focal adhesion ( red) Fuwu Zhang Zhang, F. et al ., JACS (2015), 137, 2056 − 2066 Hydrogels for injectable polymer Live fibroblast cells (green) and dead cells (red) after 20 days Kevin Wacker Xun He <Acknowledgements> - Dr. Cynthia J. Meininger and Dr. Andeea Trache for vascular endothelial cells and discussions. He, X., et al., J. Mater. - NIH PEN (HHSN268201000046C), NIH (R01-DK082546), Chem. B, 2014, 2 (46), NSF (CHE-1410272, DMR-1309724, DMR-1507429), and 8123-8130 Welch Foundation (A-0001) for financial support - LSBI for confocal imaging February 3, 2016 Imaging Science Spotlight Series

Beiyan Nan , Department of Biology, Photo-Ac.vated Localiza.on Microscopy ( PALM ) Our Microscope Your dream: Defined localizaAon Powerful lasers: Slow moAon 405 nm, 100 mW 488 nm, 200 mW 532 nm, 200 mW 561 nm, 200 mW The reality: Super spaAal resoluAon: Patchy localizaAon 64 nm or 107 nm Fast moAon Super Ame resoluAon: Up to 10 ms (100 frames/sec) The PALM soluAon: Photo-acAvaAon Unit Live imaging Single molecule tracking Contact Super spaAal resoluAon bnan@bio.tamu.edu Super Ame resoluAon

Modulation of in vivo Dynamics of Antibodies for Diagnostic Imaging Raimund J. Ober E. Sally Ward Radiology, UTSW Ralph Mason Srinivas Chiguru Rafal Swiercz Xiankai Sun Amir Tahmasbi Saleh Ramezani Ramraj Velmurugan Dilip Challa

Micro X-Ray and CT – ~25-50 μm Resolution – CT-Guided Histology SEM / EDX – Low-Vac – Maintain Sample Integrity – EDX – Elemental Composition Light Microscopy – Plastic Histology – Tissue/Device Interface – Standard ParafLin Histology vetmed.tamu.edu/labs/cvpath 979.458.1074

Biometric Tools for Decision Making Eye tracking Attention & visual behavior EEG Arousal (pupil) Cogni.ve ac.va.on Face reading GSR Emotional impact Emotional valence Emotional impact

Labo Laboratory f y for Mo r Molecular Simula2o lecular Simula2on - n - lms.c lms.chem. hem.tamu.edu amu.edu Imaging Science Spotlight Series February 3, 2016 WORKSHOPS/TRAINING SOFTWARE Linux – 3 hour lecture/3 hour hands-on session 3 BIOVIA (Accelrys) Materials Studio, Discovery Studio x year (Fall, Spring, Summer). Next: Schrödinger Solware Suite Feb. 12, 2016 CCG MOE Molecular Modeling – 3 hour lecture/3 hour hands- on session. 2 x year (Fall & Spring). Wide variety of academic and open source solware Next: Feb. 19, 2016 designed for atomis.c simula.ons. Quantum Mechanics – 6 1-hour lectures and 6 2-hour BIOVIA (Accelrys) Pipeline Pilot Imaging: hnp:// hands-on sessions over 3 days – 1 x year (Summer). accelrys.com/products/datasheets/imaging- collec.on.pdf Individualized training sessions available. Consulta.on and support for using TAMUs High Performance Research Compu.ng (HPRC) machines. HARDWARE Linux Computer Lab for training 1920-core x86_64 cluster Assistance in purchasing, installing, and trouble- shoo.ng solware. 990-core x86_64 cluster

Single Particle Analysis of Intraflagellar Transport Hongmin Qin, Biology TAMU

Optical Imaging for Tracking Metastatic Cancer and Nanoparticle Therapies Nanoparticles Xtreme In Vivo MP4 Near-IR Dye-labeled (Bruker) Nanoparticles Loaded with Paclitaxel 1 h 3 h 8 h 24 h 4 d Free dye In Vivo Biodistribution over Time Nanoparticle Imaging (Near-IR Fluorescence) Oral Intubation of Nanoparticle Suspension 1 wk 2 wk 3 wk 4 wk 5 wk Osteosarcoma Cells (GFP, Water Control Luciferase) SCKs Micelles * Shell-Crosslinked (SCKs) Paclitaxel Nanoparticles * Last panel histogram intensity reduced 50x for visualization Intra-Tibial Injection of Osteosarcoma Cells Cancer Cell Imaging (Luciferase Luminescence) Imaging Spotlight Series February 3, 2016

Ischemic Stroke and Aging Farida Sohrabji, Neuroscience and Experimental Therapeu.cs Women’s Health in Neuroscience Program Sohrabji@medicine.tamhsc.edu Stroke Therapies — Stroke is the 4 th leading cause of death and the leading cause of long term disability, with a yearly medical cost of $74 billion Scrambl miR-36 ed miR 3-3p — Approximately 55,000 more women mimic than men have a stroke each year — Women accounted for 63.0% of US Imaging: Assessment of infarct volum stroke deaths in 2005 over .me — Women also have longer hospital stays, Assessment of drug localiza. are more likely to be transferred to assisted living facili.es, and more likely to suffer post stroke depression Middle Adu Aged lt

Wesley Thompson/Biology Neuromuscular Junc.on of Adult Rat blocked for 4 days

Integrated nano-imaging techniques applied to live cell dynamics in vascular disease Andreea Trache Department of Medical Physiology, Texas A&M Health Science Center Department of Biomedical Engineering, Texas A&M University • Disease model: aging, thoracic aneurism, hypertension Confocal AFM • Cellular adap.ve responses to mechanical signaling TIRF • Func.onal coupling between adhesion strength and cell s.ffness • Integrin dependent regula.on of ac.n cytoskeleton Atomic Force Microscopy Fluorescence imaging localized mechanical stress focal adhesions and cytoskeleton Force Energy Morphology Loading S@ffness Remodeling