genovo
play

Genovo Create customized genome de novo ! Whats Genovo Genovo is a - PowerPoint PPT Presentation

Dec 24, 2022 •344 likes •812 views

Genovo Create customized genome de novo ! Whats Genovo Genovo is a Computer-Aided Design (CAD) suite to create genome in silico and guide users to synthesize and assemble it experimentally. Overview GUI Genovo Properties Interfacially,

  1. Genovo Create customized genome de novo !

  2. What’s Genovo Genovo is a Computer-Aided Design (CAD) suite to create genome in silico and guide users to synthesize and assemble it experimentally. Overview GUI

  3. Genovo Properties Interfacially, Genovo is developed on a genome browser, JBrowse.

  4. Genovo Properties Functionally, Genovo is based on GeneDesign, and BioStudio under SC2.0 project, but has great extensions.

  5. Graphical User Interface - Friendly interface Overview GUI Function

  6. GUI & Usage

  7. Function - Genovo plugins & usage GUI Function Application

  8. Modules 1 – NeoChr (Create chromosome) 2 – NucleoMod (Edit CDSs) 3 – SegmMan (Split sequence)

  9. Module 1 – NeoChr - New Chromosome Designer

  10. NeoChr NeoChr would assist users to get genes and other features to construct a new pathway then a neo-chromosome.

  11. NeoChr The operations include: • Grab genes from pathways • Rewire genes’ relation * • Define genes’ orienation and order • Decouple the genes with overlap • Add or delete features, telomere, loxp sites to build a brand new genome. * this is partially done

  12. Module 2 – NucleoMod - Sequence Modifier

  13. NucleoMod NucleoMod would allow users to edit several features of genes.

  14. NucleoMod The operations include: • Design CRISPR sites for silencing wild type genes only • Create & eliminate enzyme sites • Optimize & swap codon • Smash repeats

  15. NucleoMod 1 of four plug-ins: Design CRISPR sites for silencing wild type genes only

  16. NucleoMod 4 of four plug-ins: Smash repeats

  17. NucleoMod – Compatibility Mutiple output including GFF, FASTA, SBOL

  18. Module 3 – SegmMan - Segmentation for synthesis

  19. SegmMan • SegmMan would allow users to split chromosome into fragments in different sizes for assembly.

  20. SegmMan • Analyze the enzyme sites distribution • Split neochr into 30k megas, then 10k chunks and 2k minis for synthesis and experimental assembly

  21. Design principle

  22. Design principle

  23. Design principle

  24. Complementary – OLSDesigner - Segmentation for chip synthesis

  25. SegmMan – Complementary • For the users who synthesize DNA in chip can utilize OLS(Oligo library synthesis) Designer to design oligos and enzyme sites, primers to assemble 500-800 fragments.

  26. Application Function Application Human practice

  27. Collaboration • Cooperation: Shenzhen_BGIC_ATCG We use NucleoMod to assist other wet team, Shenzhen_BGIC_ATCG, to design the Biobrick optimized for eukaryons.

  28. Collaboration We transform the prokaryon version of cas9 for S.cerevisiae automatically. • Firstly, delete five enzymes of biobrick standard, Eco RI, Xba I, Spe I, Pst I, Not I based on synonymous mutation of CDS. • Secondly, optimize the sequence for S.cerevisiae according to codon usage database.

  29. Collaboration • Cooperation: Project SC 2.0 We tried out SegmMan module to automatically split designed yeast_chr07_4_51 into 2k minichunks in command line.

  30. Human Practice Application Human Practice Acknowledgement

  31. Human Practice Share: • Web-based server http://192.185.29.142/~luxujia/jbrowse_no_git/index.html • Software comparison with BioStudio

  32. Human Practice Share: • Host as 6 team’ regional workshop Each team shared their ideas and progress, and even established cooprative intention: SUSTC-Shenzhen-A assisted BGIC_ATCG to purchase experimental material.

  33. Human Practice Advertisement: • T-shirt sale We tried to sell our designed iGEM shirt to BGI’s employees.

  34. Acknowledgement Application Acknowledgement

  35. Team • Shenzhen_BGIC_0101 is a consortium consists of 13 co-culture students from 6 universities.

  36. Instructors & Advisors Dr. Patrick Yizhi CAI Dr. Yu CHEN Dr. Huanming YANG Mr. Kang KANG Mr. Yun WANG Miss Yue SHEN

  37. Sponsors

  38. Thank you !

  39. Supplement

  40. NeoChr Click = activation <-> repression Shift + click = change gene’s strand Double click = select genes * Node means gene * Arrow means relation Ctrl + click = change position in window

  41. NeoChr Add Delete

  42. NucleoMod 2 of four plug-ins: Create & eliminate enzyme sites

  43. NucleoMod 3 of four plug-ins: Optimize & swap codon * www.kazusa.or.jp/codon/ ‎

  44. Collaboration • Task log

  45. Collaboration • Finally, we recreate the sequence and annotation file.

  46. Collaboration Segmentation result – the fasta file of 2k minis

Recommend

More recommend