from Lippia salsa Griseb. (Verbenaceae) Florencia Antonella Musso, - PowerPoint PPT Presentation

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae) Florencia Antonella Musso, Valeria Cavallaro and Ana Paula Murray I N Q U I S U R - C O N I C E T , D E P A R T A M E N T O D E Q U Í M I C A , U N I V E R S I D A D N A C I O N A L D E L S U R , A V D A . A L E M 1 2 5 3 , B A H Í A B L A N C A , A R G E N T I N A . E - M A I L : a p m u r r a y @ u n s . e d u . a r

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae) Lippia salsa (Verbenaceae) endemic species from Argentina

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae) Ethanolic extrac + AChE inhibition IC 50 = 0.89 mg/mL

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae) • Liquid-liquid extraction Ethanolic extrac suspended in H 2 O EtOAc sub-extrac + AChE inhibition (40 % inhibition at 0.45 mg/mL)

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae) • Bioassay-guided chromatographic separation of active sub-extract Silica gel 60 hexane:EtOAc as eluent Sephadex LH20 Fractions 14-16 (110 MeOH as mg) were the most eluent active with 79.2 % of AChE inhibition at 0.45 mg/mL

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae) • Bioassay-guided purification of active fractions . . . . 17 21 22 25 2 1