Microbiological Test (Stamp Method) for Food Hygiene Microbiological Test (Stamp Method) for Food Hygiene Food Stamp Food Stamp Simple and easy prepared media for detection of contaminant from the specimen directly! Most kinds of media for your test purpose Most kinds of media for your test purpose and targeted microorganisms are available and targeted microorganisms are available
What is “ “Food Stamp Food Stamp” ”? ? What is It is simple prepared culture media that can be handled by anyone. For keeping quality and safety of the products, and also prevent food borne accident. Best solution for your routine test! You can start food hygiene test anytime, even you have never experienced microbiology!
Intended Use, Food Stamp Intended Use, Food Stamp It is useful for “Self Hygiene Control” at all kinds of food related industries. ① Test for Food Manufacturing Environment: Hygiene control for manufacturing apparatus, Kitchen apparatus (food processor, cutting board, etc.) and manufacturing environment (wall, production line, working surface, etc.). ② Test for confirmation of cleanliness, disinfection: Confirm cleanliness and disinfection. ③ Hygiene education for employees Hygiene education and enlightenment
Various kinds of Food Stamp Various kinds of Food Stamp You can select the medium by purpose and use! You can select the medium by purpose and use! There are 10 kinds of media are prepared. There are 10 kinds of media are prepared. ⑤ TCBS ① Standard method ② Desoxycholate ③ X-GAL ④ XM-G (Total viable count) (Coliform) (Coliform) (Coliform/ E. coli ) ( V. parahaemolyticus ) ⑩ Potato Dextrose with CP ⑥ TGSE ⑦ MLCB ⑧ Cereus ⑨ Sabouraud (Food poisoning Yeast and ( S. aureus ) ( Salmonella ) ( B. cereus ) (Yeast and Mold) Mold)
Food Stamp and test purposes Food Stamp and test purposes Target bacteria Name of medium Purpose of test Hygiene control for food and manufacturing Total viable count Standard method agar environment. Coliform Desoxycholate agar Hygiene control for food and manufacturing environment. X-GAL agar Control for fecal pollution by coliform Coliform / E. coli XM-G agar isolation. Test for sea food and hygiene control for its V. parahaemolyticus TCBS agar processing environment. Hygiene control for Meat / Environment and S. aureus TGSE agar Employees. Hygiene control for Egg / Meat and Salmonella MLCB agar manufacturing environment. Hygiene control for Grains / Meats / Cereus agar B. cereus Vegetables and manufacturing environment. Yeast and Mold Sabouraud agar Hygiene control for Meats and manufacturing environment. Potato Dextrose agar with CP
Operation of Food Stamp Operation of Food Stamp Cut off a side of bag, and take out Food Take off the cap of Food Stamp plate and gently press the medium against the surface of Stamp from the bag. Hold the cap of Food specimen immediately. The surface of medium / agar is elastic enough to be pressed Stamp and cut off each plate by bending. firmly against the specimen. Caution: 1. Wash or clean up the place of specimen where Food Stamp used. 2. Press against the different parts of the specimen when several kinds of Food Stamps use simultaneously. Put the cap again immediately after pressing. Put the Food Stamp plate into the incubator. Write necessity information (date/name of In case of the incubator was not available, All colonies grown on the medium surface Specimen etc.) on the cap with oil felt pen. It may be possible to keep plates at room shall be counted. A piece of Food Stamp Caution: Temperature for 1.5 – 2 times longer time. has a medium surface of 10 cm 2 . And it may Do not write any information inside of the Incubation Temp.: Incubation Time: be recommended to use the square imprinted Fungi 20-25 ℃ cap. It may hindrance to read surface of 2 – 3 days on the back of container. (1 cm 2 / square) the medium. (Sabouraud and PDA) Others 35-37 ℃ 1 – 2 days XM-G 35 ℃ 20 ± 2hours.
Interpretation of Food Stamp (1) Interpretation of Food Stamp (1) Name of medium Interpretation ① Standard Method agar Count all colonies grown on the surface. ② Desoxycholate agar Red colonies (Coliforms) ③ X-GAL agar Blue Green-Blue colonies (Coliforms) ④ XM-G agar Blue colonies ( E. coli ) / Red colonies (Coliforms) ① Standard Method agar ② Desoxycholate ③ X-GAL ④ XM-G (Total viable count) (Coliform) (Coliform) (Coliform/ E. coli ) Mainly used for controlling of fecal pollution by coliform isolation.
Interpretation of Food Stamp (2) Interpretation of Food Stamp (2) Name of medium Interpretation ⑤ TCBS agar Green colonies ( V. parahaemolyticus ) Note1 ⑥ TGSE agar Black colonies with an opaque ring around these colonies ( S. aureus ) ⑦ MLCB agar Black and/or black center colonies ( Salmonella ) Note2 ⑧ Cereus agar White, slightly thick colonies with an irregular rim Note1: V. alginolyticus is not pathogenic, their existence, if too many yellow colonies present, implies a risk of contamination by V. parahaemolyticus . Note2: Citrobacter or other organisms may also develop black colonies just like Salmonella . It is recommended that certain identification tests should be performed simultaneously. ⑧ Cereus ⑤ TCBS ⑥ TGSE ⑦ MLCB ( B. cereus ) ( V. ( S. aureus ) ( Salmonella parahaemolyticus ) ) Food poisoning bacteria (basically it should not be presented in the products)
Interpretation of Food Stamp (3) Interpretation of Food Stamp (3) Name of medium Interpretation ⑨ Sabouraud agar Count all fungi grown on the surface ⑩ Potato Dextrose agar with CP Count all fungi grown on the surface (bacteria inhibit growth) Mold Yeast ⑨ Sabouraud ⑩ Potato Dextrose with CP (Fungi) (Food poisoning Yeast and Mold) Use these plates to detect fungi as contaminant
Egg Yolk Reaction of Staphylococcus aureus Staphylococcus aureus Egg Yolk Reaction of Either one of two egg yolk reactions is positive, Identify those colonies are Staphylococcus aureus . Lecithinase Reaction Lipase Reaction Lecithinase Reaction Lipase Reaction It is observed “Pearly luster” on the surface of It is observed “Turbid” inside of medium medium around colonies from sidelong glance. around colonies from top surface.
Judgment of Cleanliness by the number of colonies isolated Judgment of Cleanliness by the number of colonies isolated ● Quantitative test: Judged by the number of colonies isolated (Contaminants) Colonies/plate Judgment Mark Cleanliness 0 ～ 9 ◎ － or ± Negative / less contamination 10 ～ 29 〇 ＋ Slight contamination 30 ～ 99 △ ① Standard method ② Sabouraud ③ Potato Dextrose ＋＋ Moderate contamination (Total viable count) (Yeast and Mold) with CP (Food poisoning × Over 100 ＋＋＋ Severe contamination Yeast and Mold) ● Qualitative test: Judged by (Food poisoning bacteria) ① Desoxycholate ③ XM-G ⑤ MLCB ⑦ Cereus (Coliform) (Coliform/ E. coli ) ( Salmonella ) ( B. cereus ) ② X-GAL ④ TCBS ⑥ TGSE ） (Coliform) ( V. parahaemolyticus) ( S. aureus )
Example of use, Food Stamp (1) Example of use, Food Stamp (1) Hygiene check (kitchen apparatus) Total Viable Count Coliforms S. aureus Cutting Kitchen Cooking Dishcloth Sponge board Knife table
Example of use, Food Stamp (2) Example of use, Food Stamp (2) Check for cleaning, sterilization Cooking table Total Viable Count Coliforms S. aureus After After After Before spraying of washing washing by Ethanol washing by water Detergent
Example of use, Food Stamp (3) Example of use, Food Stamp (3) Check for Hand washing (for employees education) Mr. MR Ms. TS Hand Washing Total Viable Count Coliforms S. aureus Before After After Before
Example of use, Food Stamp (4) - - 1 1 Example of use, Food Stamp (4) Hygiene control for food / material (Sea Food) Food Stamp application for Sea Food Total Viable Count Coliforms Vibrio parahaemolyticus Tuna Scallop Prawn Garnish Ark shell (Radish)
Example of use, Food Stamp (4) - - 1 1 Example of use, Food Stamp (4) Hygiene control for food / material (Daily dishes) Food Stamp application for Daily Dishes Total Viable Count Coliforms S. aureus Sandwich Sandwich Rice Ball Tofu Salad (Lettuce) (Ham) (Rice) (soybean curd )
Direction for Use (1) Direction for Use (1) Isolation data will be changed where the places stamped. Refrigerator Total Viable Count Coliforms S. aureus Top shelf Second Internal Doorknob shelf Surface
Direction for Use (2) Direction for Use (2) Isolation data will be changed when Food Stamp used. Cooking table Total Viable Count Coliforms S. aureus Before work During work After washing
Adaptable test objects, Non adaptable Adaptable test objects, Non adaptable objects for Food Stamp objects for Food Stamp 1) Adaptable objects: ・ Kitchen apparatus (cutting board / kitchen knife / dish cloth, etc.) ・ Cooking table, Work bench, Wall, Floor ・ Instruments (Slicer / Refrigerator, etc.) ・ Employee’s Hands ・ Other (include foods that can be stamped) 2) Non adaptable objects: ・ Liquid surface ・ Not smooth and irregular surfaces ・ Acquire lots of oil on the surface (thin film of oil will not be the problem)
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