Exploitation of PLGA conjugates in drug delivery Francesca SELMIN, - PowerPoint PPT Presentation

Jul 23, 2023 •152 likes •282 views

Exploitation of PLGA conjugates in drug delivery Francesca SELMIN, PhD MIPOL Milan Polymer Days February 15-16, 2017 Implants are non -conventional drug delivery systems for parenteral implantation. Definition (Eur. Pham. 9 th ed

Exploitation of PLGA conjugates in drug delivery Francesca SELMIN, PhD MIPOL – Milan Polymer Days February 15-16, 2017
Implants are… … non -conventional drug delivery systems for parenteral implantation. Definition (Eur. Pham. 9 th ed 2017) … sterile, solid preparations of a size and shape suitable for parenteral implantation and release of the active substance(s) over an extended period of time. Each dose is provided in a sterile container.
Implants are… … non -conventional drug delivery systems whose performances have to be guaranteed in terms of Quality Safety Efficacy
Critical attributes Quality • ↓ Loading capacity Biocompatibility • ↓ Encapsulation efficiency Biodegradability • ↓ Chemical stability Processability • ↓ Drug release Mechanical stability
Poly(lactide-co-glycolide) ↑ Approved ↓ Limited loading capacity ↑ Biocompatibility ↓ Low encapsulation efficiency ↑ Biodegradability ↓ Low stability upon sterilization ↑ Processability ↑ Mechanical stability ↓ pH decrease into the  -enviroment 2h 7d @ the administration site Semin Immunol 20 (2008) 86 – 100 J. Control. Rel. 101 (2005) 163 – 173
Sterilization of PLGA based DDS and medical devices H abstraction In presence of oxygen @ room temperature Chain scission Montanari et al. / J Control Rel 56(1998):219-229
Anti-oxidant properties PLGA conjugates: g-AA-PLGA 2) AA PLGA Anti-oxidant grafted PLGA DPPH assay (% inhibition) AA moiety Polymer 1h 2h 3h 24h -- PLGA 0 0 0 0 Pyrogallic acid g-PA-PLGA 76 77 81 98 Caffeic acid g-CA-PLGA 92 93 93 98 Resveratrol g-RV-PLGA 27 32 37 56
Stability after  -sterilization PLGA conjugates: g-AA-PLGA Before irradiation After irradiation PLGA g-PA-PLGA PLGA g-PA-PLGA Mn (kDa) 12.1±0.0 9.2±0.1 8.9±0.3 9.2±0.2 Mw (kDa) 17.3±2.9 16.3±0.1 15.2±0.2 15.9±0.2 PI 1.4±0.2 1.8±0.1 1.8±0.01 1.6±0.02 T g (°C) 42.4±0.5 43.7±0.0 40.7±0.0 41.9±0.2  g-CA-PLGA  Irradiated g-CA-PLGA Cilurzo et al. / PAT 2010(22):2201-2205
pH variation during degradation PLGA conjugates: g-PA-PLGA PLGA g-PA-PLGA Cilurzo et al. / PAT 2010(22):2201-2205
In vitro release of a proteic drug PLGA conjugates: g-CA-PLGA D 50 (  m)  EE% (mV) 13.8  0.0 -29.8  1.3 PLGA - 13.8  1.1 -24.8  1.4 g-CA-PLGA - 13.1  0.9 -11.2  3.0 PLGA 35±0.7 15.4  1.3 -15.1  1.1 g-CA-PLGA 95.6±2.7 Selmin et al. / J Funct Biomat 2015(6):1-13
Stability after  -sterilization PLGA conjugates: g-CA-PLGA Selmin et al. / J Funct Biomat 2015(6):1-13
Acknowledgment @DisFarm - UniMI Francesco CILURZO Paola MINGHETTI Giulia MAGRI Luisa MONTANARI @DisFEB - UniMI Stefano BELLOSTA Silvia CASTIGLIONI @uniCAL Francesco PUOCI

Recommend

SELF ASSEMBLED NANOSTRUCTURE OF PLGA- GLUCOSAMINE COMPOSITE NANOPARTICLES M. Marimuthu 1 , S. Kim

18 TH INTERNATIONAL CONFERENCE ON COMPOSITE MATERIALS SELF ASSEMBLED NANOSTRUCTURE OF PLGA- GLUCOSAMINE COMPOSITE NANOPARTICLES M. Marimuthu 1 , S. Kim 1 * 1 College of Bionanotechnology, Kyungwon University, Gyeonggi do, Republic of Korea *

430 views • 4 slides

! Current State of Exploitation ! Return-Oriented Exploitation ! Mac OS X x86 Return-Oriented

! Current State of Exploitation ! Return-Oriented Exploitation ! Mac OS X x86 Return-Oriented Exploitation ! Techniques ! Demo ! Mac OS X x86_64 ! Conclusion ! Morris Worm (November 1988) ! Exploited a stack buffer overflow in BSD in.fingerd on VAX

1.02k views • 80 slides

Trimethoxyphenyl Conjugates Kevin D. OShea 1 , Michael M. Cahill 1 , Larry T. Pierce 1 ,

Synthesis and Anticancer Activity of Novel Indole- Trimethoxyphenyl Conjugates Kevin D. OShea 1 , Michael M. Cahill 1 , Larry T. Pierce 1 , Florence O. McCarthy 1,* 1 School of Chemistry and ABCRF, Cavanagh Building, University College Cork,

539 views • 24 slides

of quinoline-antimicrobial peptide conjugates as antibacterial drugs Pierre Laumaill 1* ,

Synthesis, biological evaluation and membranotropic properties of quinoline-antimicrobial peptide conjugates as antibacterial drugs Pierre Laumaill 1* , Alexandra Dassonville-Klimpt 1 , Sophie Da Nascimento 1 , Catherine Mulli 1 , Franois

517 views • 23 slides

$Rauzy fractals and Equi-distribution of Galois- and beta-conjugates of Parry Numbers near the$

Rauzy fractals and Equi-distribution of Galois- and beta-conjugates of Parry Numbers near the

Rauzy fractal and equi-distribution of Galois- and beta-conjugates of Parry Numbers near the unit circle Rauzy fractals and Equi-distribution of Galois- and beta-conjugates of Parry Numbers near the unit circle Jean-Louis Verger-Gaugry Prague,

1.07k views • 54 slides

Quantum Dot Conjugates for Imaging Applications Sungjee Kim Dept. of Chemistry POSTECH QD as

Quantum Dot Conjugates for Imaging Applications Sungjee Kim Dept. of Chemistry POSTECH QD as Bright & Tunable IR Emitter Slide 1 Lanthanide complexes (2) Organic dye molecules (1) - Low quantum yield at NIR & IR range - Limited

554 views • 27 slides

Department of Mathematics University of Manitoba Canada On Semigroups Admitting Conjugates G.I.

Department of Mathematics University of Manitoba Canada On Semigroups Admitting Conjugates G.I. Moghaddam , R. Padmanabhan Groups St Andrews 2017 August 7, 2017 Introduction While every subsemigroup of a group is cancellative, a famous

485 views • 19 slides

Computational Systems Pharmacology of Antibody-Drug Conjugates: A Joint Academia-Industry

Computational Systems Pharmacology of Antibody-Drug Conjugates: A Joint Academia-Industry Experience Inez Lam IMAG-AND Futures Meeting March 17, 2020 Mac Gabhann Lab at Johns Hopkins University C COMPUTATIONAL DESIGN of THERAPEUTICS LAB

385 views • 20 slides

Z -basis for the orders generated by the conjugates of algebraic integers St ephane R.

Z -basis for the orders generated by the conjugates of algebraic integers St ephane R. LOUBOUTIN Aix Marseille Universit e, CNRS, Centrale Marseille, I2M, Marseille, FRANCE stephane.louboutin@univ-amu.fr June 26, 2018 Contents 1

403 views • 17 slides

Weird Machines on Little Robots Intro to binary exploitation on Android smartphones @f0rki

Weird Machines on Little Robots Intro to binary exploitation on Android smartphones @f0rki 2013-06-06 Agenda Motivation ARM Primer Exploitation 101 Science, Bitches! Vulnerability classes Exploitation Defenses & Mitigation Techniques

899 views • 51 slides

Passive immunotherapy: antibody drug conjugates Anti CD-79b: Polatuzumab Maurizio Martelli Dip.

Passive immunotherapy: antibody drug conjugates Anti CD-79b: Polatuzumab Maurizio Martelli Dip. Biotecnologie Cellulari ed Ematologia Sapienza Universit di Roma Polatuzumab vedotin: CD79b target Molecular Informa.on CD79b

615 views • 42 slides

An Introduction to Elder Abuse for Professionals: Financial Exploitation NCEA Financial

An Introduction to Elder Abuse for Professionals: Financial Exploitation NCEA Financial Exploitation 1 Learning Objectives At the end of this presentation, you will be able to: Define and describe financial exploitation Identify

386 views • 34 slides

exploitation initiatives Monday, 10 December 2018 Biowaste treatment and exploitation SYMBIOSIS

CluBE and biowaste exploitation initiatives Monday, 10 December 2018 Biowaste treatment and exploitation SYMBIOSIS Sensitization Event Nikolaos Ntavos Cluster Manager n.ntavos@clube.gr Cluster of Bioenergy & Environment Formally

632 views • 37 slides

WiFi Exploitation: How passive interception leads to active exploitation SecTor Canada Solomon

WiFi Exploitation: How passive interception leads to active exploitation SecTor Canada Solomon Sonya @Carpenter1010 The problem always seems to become more tractable when presented with the solution Solomon Sonya @Carpenter1010 What to

680 views • 63 slides

CS-527 Software Security Exploitation Asst. Prof. Mathias Payer Department of Computer Science

CS-527 Software Security Exploitation Asst. Prof. Mathias Payer Department of Computer Science Purdue University TA: Kyriakos Ispoglou https://nebelwelt.net/teaching/17-527-SoftSec/ Spring 2017 Exploitation: Context/Disclaimer In this module

411 views • 37 slides

Bypassing Memory Protections: The Future of Exploitation Alexander Sotirov alex@sotirov.net

Bypassing Memory Protections: The Future of Exploitation Alexander Sotirov alex@sotirov.net About me Exploit development since 1999 Research into reliable exploitation techniques: Heap Feng Shui in JavaScript Bypassing browser

1.1k views • 48 slides

Exploitation techniques for NT kernel Introduction General concepts Internals Adrien

Exploitation techniques for NT kernel Adrien Adr1 Garin Exploitation techniques for NT kernel Introduction General concepts Internals Adrien Adr1 Garin Exploitation Stack overflow Integer overflow Write What Where EPITA

623 views • 47 slides

Organizational Structure, Exploration, and Exploitation on the ELICIT Experimental Platform Allan

Organizational Structure, Exploration, and Exploitation on the ELICIT Experimental Platform Allan Friedman Ethan Bernstein David Lazer Harvard ELICIT Outline Exploration & Exploitation ELICIT overview Extensions to ELICIT

585 views • 29 slides

Tactical Exploitation Tactical Exploitation the other way to pen-test the other way to

Tactical Exploitation Tactical Exploitation the other way to pen-test the other way to pen-test hdm / valsmith hdm / valsmith Black Hat USA 2007 Black Hat USA 2007 Las Vegas August 2007 who are we ? who are we ? H D

1.02k views • 79 slides

Spotting Red Flags of Elder Financial Exploitation in your Institution October 27, 2016 ACAMS

Spotting Red Flags of Elder Financial Exploitation in your Institution October 27, 2016 ACAMS Greater Philadelphia Chapter AGENDA Evolution of Elder Abuse/Financial Exploitation Recommendations Red Flags Case Scenarios

271 views • 16 slides

Java Card Applet Firewall Java Card Applet Firewall Exploration and Exploitation Exploration and

Java Card Applet Firewall Java Card Applet Firewall Exploration and Exploitation Exploration and Exploitation Wojciech Mostowski and Erik Poll Digital Security Radboud University Nijmegen The Netherlands http://www.cs.ru.nl/~{woj,erikpoll}/

818 views • 42 slides

Israels Exploitation of Palestinian Natural Resources Suha Jarrar Environmental Policy

Israels Exploitation of Palestinian Natural Resources Suha Jarrar Environmental Policy Researcher Al-Haq 2018 How exploitation of natural resources drives the illegal occupation The Occupied Palestinian Territory (OPT) is rich in

1.12k views • 13 slides

Exploitation of instruction level parallelism Computer Architecture J. Daniel Garca Snchez

Exploitation of instruction level parallelism Exploitation of instruction level parallelism Computer Architecture J. Daniel Garca Snchez (coordinator) David Expsito Singh Francisco Javier Garca Blas ARCOS Group Computer Science and

689 views • 55 slides

Dienstag 30. Nov. 10 [] All Your Baseband Are over-the-air exploitation of memory corruptions in

Dienstag 30. Nov. 10 [] All Your Baseband Are over-the-air exploitation of memory corruptions in GSM software Ralf-Philipp Weinmann Laboratory for Algorithmics, Cryptology & Computer Security University of Luxembourg

901 views • 46 slides