BI OREACTORS NEW SOLUTI ONS FOR OLD PROBLEMS Dr. Pavel Lehky - PowerPoint PPT Presentation

BI OREACTORS NEW SOLUTI ONS FOR OLD PROBLEMS Dr. Pavel Lehky LAMBDA – Laborat ory I nst rument s www.rhone.ch/ lambda 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

OUTLI NE • St irring mechanism • I R-heat ing syst em • Approach used in t he MI NI FOR • Probes • Ferment at ion accessories (Pumps and gas f low cont roller) • Conclusions 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

TRADI TI ONAL STI RRI NG a) Mechanical seals • No physical separat ion • Cont aminat ions • Service “sensit ive” 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

TRADI TI ONAL STI RRI NG b) Magnetic coupling • Separat ion � St erilit y • Dead spaces • High cost 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

NEW STI RRI NG SCHEME Up and down movement 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

NEW STI RRI NG SCHEME Fermentor head • Separat ion � St erilit y • Elect romagnet � Mot or • Easy t o mount (one hand) • Threaded necks • Low cost 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

NEW STI RRI NG SCHEME Stirring discs • Count er rot at ing f low pat hs • Vert ical st ream (t hrough t he holes) 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

NEW STI RRI NG SCHEME Stirring properties • Whole volume st irring • Good gas dist ribut ion • No vort ex f ormed • No baf f les needed • Reduced f oaming • Gent le t o cells 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

NEW STI RRI NG SCHEME I n action… 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

AI R SPARGERS b) Silicone a) Capillary • No blocking by salt s Possible blocking by salt s • Good bot t om circulat ion and bubble breaking 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

FERMENTATI ON VESSELS • Adopt ed f rom cell cult ure • Up t o 8 t hreaded side necks • Good accessibilit y • Variabilit y • Low cost • “j acket able” 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

STOPPER • Ef f icient sealing • Reusable many t imes 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

“COFFEE CUP” RADI ATOR • I R-heat ing � gent le sun-like heat ing • Nat ural convect ion • No cont act wit h vessel • No overheat ing at any volume • Up t o 60°C in 3l vessel • 98% energy yield • Negligible heat capacit y • Low volt age 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

THE MI NI FOR APPROACH Fermentor case • I nt egrat ed � f ree-st anding vessel • Cascaded design • Ergonomic arrangement • Excellent accessibilit y • Ext remely compact 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

THE MI NI FOR APPROACH Control panel • Closest t o operat or • All inf ormat ion at a glance (no scrolling or menu select ion) • Easy operat ion 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

THE MI NI FOR APPROACH Radiator • Sealing vessel holder � spill prot ect ion • Vessels above heat source • Shielding of t he hot coil � prot ect ion against burning 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

THE MI NI FOR APPROACH Front connectors • Short est connect ion bet ween probes and vessel • Sealed cables 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

THE MI NI FOR APPROACH Fermentor platf orm • Air out put and st orage bot t les behind t he vessel • Short est connect ion bet ween pumps and vessel • Magnet ic holders 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

THE MI NI FOR APPROACH Rear side 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

THE MI NI FOR APPROACH General view • Up t o 4 pumps on support s • Pump prot ect ion in case of t ubing rupt ure • Good visibilit y of pump st at us • Easy accessibilit y f rom all sides 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

THE MI NI FOR APPROACH Serial f ermentation • Opt imisat ion in series • Can be used separat ely (complet ely independent ) 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

PROBES pH and temperature probe • Variopin connect or (f reely st erilisable) • Pt 100 int egrat ed in t he glass elect rode 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

PROBES DO probe • PEEK body • Freely st erilisable connect or • Large cat hode syst em 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

PROBES DO probe membrane module • Glass support ed PTFE membrane • Fast response • Membrane prot ect ion 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

I NOCULATI ON & SAMPLI NG • St ainless st eel capillaries • Luer-Lock adapt ers 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

PERI STALTI C PUMPS • Const ant compression • Compact cont rol • Reliable • Large rollers • Economy of t ubing • Asymmet ric head • Progressive speed cont rol 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

PERI STALTI C PUMPS I n action… 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

MASSFLOW Gas f low control • Cont rolled aut omat ic addit ion of CO 2 or N 2 during cell cult ures • No need of expensive gas st at ions • Programmable • Tot aliser 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

CELL CULTURE ADAPTOR • Very gent le st irring • Oxygenat ion t hrough moving coil 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

I NTEGRATOR • Tot alisat ion of delivered liquid or gas • Access t o growt h kinet ics • Enzyme act ivit y measurement 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

FERMENTATI ON Addit ion of acid or base necessary t o keep t he pH const ant would not appear wit hout PRECI FLOW and I NTEGRATOR ! 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

FNet – SOFTWARE (Sysmatec) • Complet e easy t o use sof t ware f or • Dat a saving laborat ory f erment ors • Graph scaling • Connect ion of up t o 6 f erment ors, • Prof ile def init ion 12 int egrat ors and 6 pumps • Alarms 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

CONCLUSI ONS • A maj or ef f ort was invest ed t o make bioreact ors more ef f icient and simple • New up and down st irring concept providing easy st erilit y • I R-heat er f or gent le heat ing • pH and DO probes • Pumps and gas f low cont roller 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda

This work would not have been possible without: Jaroslav Bernášek, Vladimir � ermák, Rainer Glöckler, Vlastimil Hanuš, Hans Kulla, Lida Lehká, Jan Marc Lehky, Petr Lehky, Stanislav T � íska, Daniel Venetz, Patrick Yax, Michal Zemek THANK YOU !!! 1 st I nternational Congress on Bioreactor Technology www.rhone.ch/ lambda