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Aug 09, 2023 •252 likes •408 views

Bacto - Art A Multicomponent Inducible Expression System for Teaching and Discovery North Carolina Agricultural and Technical State University North Carolina Agricultural and Technical State University What is Bacto Art? www.ncat.edu

  1. “Bacto - Art” A Multicomponent Inducible Expression System for Teaching and Discovery North Carolina Agricultural and Technical State University

  2. North Carolina Agricultural and Technical State University What is Bacto Art? www.ncat.edu

  3. North Carolina Agricultural and Technical State University Who are we Targeting Our Primary target is Middle and High  school students www.ncat.edu

  4. North Carolina Agricultural and Technical State University Project Goals » Create an inducible expression system » Hands-on experience in the fields of molecular and synthetic biology www.ncat.edu

  5. North Carolina Agricultural and Technical State University Construction of Component: 3A Assembly www.ncat.edu

  6. North Carolina Agricultural and Technical State University Construction of Component: 3A Assembly IPTG Xyl www.ncat.edu

  7. North Carolina Agricultural and Technical State University Testing the Component: Function www.ncat.edu 7

  8. North Carolina Agricultural and Technical State University Testing the Component: Function www.ncat.edu 8

  9. North Carolina Agricultural and Technical State University SDS-PAGE Analysis www.ncat.edu 9

  10. North Carolina Agricultural and Technical State University Applications: BactoArt as a Teaching Tool 1. Students “ Paint On ” Inducer www.ncat.edu

  11. North Carolina Agricultural and Technical State University Applications: BactoArt as a Teaching Tool 2. Plate BactoArt Bacteria www.ncat.edu

  12. North Carolina Agricultural and Technical State University Applications: BactoArt as a Teaching Tool 4. Excite FPs using Widefield Illumination 5. Discuss Regulated Gene Expression & Transcription/ Translation www.ncat.edu

  13. North Carolina Agricultural and Technical State University Future Directions  Continue developing the project so that the E. coli strain used can express, at a minimum, four different colors. » Other Fluorescent Proteins will include: • Cyan FP (CFP), Far-Red FPs (FRFP), etc.  Create a system that can make fine detailed art pictures. » Using an inkjet printer, put sugars in a cartridge to print out the desired picture www.ncat.edu

  14. North Carolina Agricultural and Technical State University Future Directions  Create photoswitchable FP Bacto-Art » Moving Pictures through incorporation of photoswitchable FPs, such as Dronpa, which can be turned on and off with different wavelengths of light. UV light Blue Light www.ncat.edu 14

  15. North Carolina Agricultural and Technical State University Acknowledgements  Mentors: Dr. H. Newman & Dr. K. Bernot  The “ Greensboro-Aggies ” iGEM team members.  This work was supported by: » I 3 /iBLEND (NSF #1038160) » RISE (NIH #R25GM076162) » The GSK Foundation. www.ncat.edu 15

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