Autors: Luiza Nicolosi Guido*, Camile Castilho Fonteles, Mariana - PowerPoint PPT Presentation

Note: for non-commercial purposes only Paternal consumption of a selenium-deficient diet programs the susceptibility of female offspring to mammary carcinogenesis Autors: Luiza Nicolosi Guido*, Camile Castilho Fonteles, Mariana Papaléo Rosim, Fabia Andrade de Oliveira, Silvia Maria Franciscato Cozzolino, Thomas Prates Ong. * Phd student at the Food Science program – Laboratory of Nutrigenomics and Programming; Department of Food and Experimental Nutrition - University of São Paulo – Brazil

Breast cancer incidence and mortality worldwide

Paternal programming Male germinative cell Fetal development in early life Risk of developing Non-communicable diseases in adulthood CARONE et al. Cell, v. 143, p. 1084 – 1096, 2010; SHEAU-FANG et al. Nature, v. 467, p. 963 – 966, 2010; RANDO. O.J. Cell, v. 151, n. 4, p. 702-708, 2012

Selenium Essential for the metabolism Implication on male fertility Protective activity against breast cancer Source of Se reported ALATISE & SCHRAUZER. Biol Trace Elem Res, v. 136, p. 127 – 139, 2010. WHANGER, P.D. Brit J. Nutr, v. 9, p. 11 – 28, 2004.

Average Selenium Intake FAIRWEATHER-TAIT et al. Antioxidants & Redox Signaling, v. 14, n. 7, 2011

Paternal breast cancer programming Male germinative cell Breast cancer risk in Mammary gland development adult life Se deficiency or in early life supplementation

Experimental design – Age in weeks Approved by the ethics committee on animal use of the University of Sao Paulo – Protocol CEUA/ FCF/ 382

Paternal data * Different compared to control; a – different compared between groups; # - marginal difference (ANOVA) – mean ± SEM p≤0,05

Female offspring data * Different compared to control; a – different compared between groups; # - marginal difference (ANOVA) – mean ± SEM p≤0,05

Mammary epithelial growth End of epithelial Limphy-node tree End of epithelial tree Nipple * Different compared to control; a – different compared between groups p ≤ 0,05 ; # - marginal difference

Mammary tumor incidence and latency p ≤ 0.05 Frequency of animals without tumor , a * Days after DMBA administration Mammary tumor incidence (first tumor appearance) of the female offspring from deficient (0.05ppm), control (0.15ppm) and supplemented (1ppm) diet fathers. p ≤ 0.05 according to ANOVA. Values expressed in mean ± SEM * Different compared to control; a – different compared between groups; # - marginal difference p≤0,05

Conclusion and Further Steps The preliminary results show that paternal selenium deficiency increases the susceptibility of the female offspring to mammary carcinogenesis, while selenium supplementation has a protective effect. Further steps will attempt to elucidate the molecular and epigenetic mechanisms involved in this programming.

Acknowledgment • Laboratory of Nutrigenomics and Programming – FCF/USP • Laboratory of Micronutrients – FCF/USP • Laboratory of Nutrition and Cancer – FCF/USP Camile C. Fontelles Mariana P. Rosim • Laboratory of Functional Food Development – FCF/USP Fábia O. Andrade Raquel S. Cruz Finantial Support: Thomas P. Ong Vanessa C. Pires Gabriela Costa