ABC IAC April 21, 2016
4MGEN14 contamination Sleuthing the problem
4MGEN14 seed overwinter Lewisetta Rap Lynnhaven 5K 5K 5K split on site split on site split at VIMS 2n 14 10 2015 distribution
4MGEN14 seed overwinter Lewisetta Rap Lynnhaven 5K 5K 5K split on site split on site split at VIMS 2n 14 10 58%
2016 CERTIFICATIONS to date A B C D E F 4MGEN13 — 40/41 23/25 — — 20/20 98% 92% 100% 4MGEN14 30/32 — 14/15 43/43 21/23 5/7 80% 93% 100% 91% 71% All certs so far 2016 176/186 (95%) 2015 345/375 (92%) 2014 327/332 (99%) 2013 371/385 (96%) 2012 428/480 (89%)
Brood stock quality Industry based research
Glycogen and Fecundity • NIRS technology • Oyster Seed Holdings • LOLA, DEBY.13, DEBY.14 • Glycogen at time zero and just prior to conditioning – Different treatments prior to conditioning • Fecundity/egg counts on spawning day
Growth
Egg count vs. Glycogen
Egg Count vs. Length
False winter and Dermo • Belief that false winter aids in conditioning and “resets” Dermo if present • H° : oysters put through false winter will have lower prevalence and intensity of Dermo compared to those not put through false winter
DEBY.LYN.13
Mackin Scale 0 – rare 1 – very light 2 – light 3 – moderate 4 – moderate/heavy 5 – heavy
RFTM results false wintered no false winter 72% prevalence 68% prevalence weighted prevalence: weighted prevalance: 1.28 (n=25) 1.32 (n=25 No difference between treatments
Update on status of VIMS research hatchery No planning until: July 1, 2017 / Start building: July 1, 2018
building concept concept views nort h
building concept concept views
design . entrance perspective
int erior design. interior perspectives- lobby
int erior design. interior perspectives- transition
int erior design. interior perspectives- lower level Interior Perspectives
int erior design. interior perspectives- lower level
revised f irst second plan . programming floor plans- level 2
revised f irst f loor plan . programming floor plans- level 1
North side
South side
Hatchery floor
Questions about the planning process What role industry? • Determining research capabilities • e.g., carbonate chemistry? • e.g., conditioning studies? • e.g., closed systems? Research hatchery manager? • Oversee facility, part of AQ Program • PI for hatchery related research • “Journeyman” with experience • e.g., Alan Barton Expertise in design? • VA industry? • Other sources of expertise • Donaldson • Meritt • Davis
Family deployment 2016
2N Deployment 2016 • 49 low salinity families – 25 also deployed in York River* • 50 high salinity families – 15 also deployed in Lewisetta* * some families perform well in both environments • long line – York River & Cambridge • rack & bag – Lynnhaven & Lewisetta
2N Deployment 2016 • Hungars Creek – Cherrystone – 30 families (15 high, 15 low, 2 reps per) – rack and bag – Do ABC families perform differently in the creek environments of the Eastern Shore? • loggers in place to measure temp. & salinity
3n mortality research update Grad student: Joseph Matt Post doc: Eric Guévélou
“The relationship between reproduction and mortality in triploid Crassostrea virginica : a matter of economic importance” Spawned in March 2015 – all groups now “ideal” size tetraploid diploid male 4n VA 4n LA 2n VA 2n ME 2n VA VVV LLV VV MV female 2n ME VVM LLM VM MM 4 triploid groups 4 diploid “controls”
Deployment sites Control
Data from triploid mortality 2016 • Triploid reproductive effort • Environment • Physiology • Growth • Mortality • Condition • NIRS • Glycogen • (Lipid)
Microbiome – BK Song, Allen, Carnegie Profiling the microbiome composition of hatchery reared oyster larvae Sea Grant PD funds Summer 2015 The totality of microorganisms and their collective genetic material present in a particular environment.
Sampling ontogeny across hatcheries
Sampling seasonal variation across hatcheries march april may june august july GP KAC OSH WOC
0.3� D-3 D-2 V-3 D-1 0.2� PC1 - Percent Variation Explained – 7.9% D-3 D-1 GP V-2 KAC V-2 0.1� D-2 D-3 PV-3 PV-2 WOC V-3 V-1 D-2 0� D-3 -0.4� PV-3 -0.3� -0.2� -0.1� 0� 0.1� 0.2� 0.3� D-1 V-1 D-1 V-1 -0.1� PV-1 PV-2 V-1 PV-3 PV-1 -0.2� D-2 V-3 PV-2 OSH -0.3� V-2 -0.4� V-3 -0.5� PC1 - Percent Variation Explained – 9.7%
D-stage V-stage Predominant genus found in adult PV-stage
New crop of OATs Other training
2016 OATs (32 applicants) 3 ‘regular’ OATs • Imani Black • Track record with Bay interests • Lacrosse star, ODU • Graduates May ‘16 • Lizzy Profita • Experience in shellfish, algae • Americorps – Cape Cod shellfish • Prefers field to lab • Haley Ladeau • Experience at Milford • Experience at AIC (Rutgers, Cape May) • Extrovert! 1 ‘research assistant’ OAT • Chris Rubino • Internships at several hatcheries • Familiar with the field • Not afraid of “unflattering jobs”
2016 OAT applicants (placements) • Nick Booth – Cherrystone intern • Carlos Santos – Mobjack Bay Seafood • James McCarthy – Lynnhaven • Ellis Chapman – Cherrystone • Diamond in the rough • Gretchen Davis – Northern Neck
2016 OAT Jr (test program) MathScience Innovation Center, Richmond • 2 week summer aquaculture course – “Tank to Table” • Tilapia • Trout • Oysters • Select four top students (work ethic) – oyster internship • 2 to VIMS for one week • Another 2 to VIMS for one week
VIMS Aquaculture Program Industry Advisory Committee redefined?
Succession at ABC (first shown June 2016) pre-2015 2015 “five” years FTE Aquaculture @ VIMS Stan Stan Jess (asst. director) Jess Jess GP KAC 2n KAC ABC field field 2n 4n GP 4n
INFRASTRUCTURE – VIMS Aquaculture Program AP Director • Brings research interest • Coordinates aquaculture research at VIMS • Administers rapid response funds Ryan Carnegie Kim Reece ABC Iris Anderson BK Song Juliette Smith Elizabeth Shadwick Jan McDowell Roger Mann
ENDOWMENT – VIMS Aquaculture Program $$ $ AP Director • Brings research interest • Coordinates aquaculture research at VIMS $ • Administers rapid response funds Discretionary funding Ryan Carnegie Kim Reece ABC Iris Anderson BK Song Juliette Smith Elizabeth Shadwick Jan McDowell Roger Mann
Shellfish Industry Advisory Committee – VIMS Aquaculture Program $$ $ AP Director • Brings research interest • Coordinates aquaculture research at VIMS $ • Administers rapid response funds Discretionary funding Ryan Carnegie Kim Reece ABC Iris Anderson BK Song Juliette Smith Elizabeth Shadwick Jan McDowell Roger Mann
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