- an antibody based biosensor
• HEWL, a suitable first candidate • Easily customized for other antigens
STEP 1: STEP 2: STEP 3: Antibody complex + Food sample False antigen bound to RFP Luciferin
Antigen-antibody binding results in green luminscence Egg-antigen
False antigen binds to the remaining unbound antibodies Due to FRET mechanism the color of the sample changes
No antigen Antigen detected present False antigen I T
Intensity Time
• Arduino micro processor • True color photosensor • Electronically amplified signal • 3D-printed case
We are so hungry, We need to find some food!
Food!
• Succesfully started a new iGEM team • Modelling • Helped other teams • Increased knowledge about synbio • Constructed a working biosensor • Designed new biobricks
Egg-antibody
Egg-antigen
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